Pore size relates to the filter's ability to filter out particles of a certain size. Although all membranes are rated for a particular pore size, pore size ratings alone are an unreliable measure of filter effectiveness because these ratings vary from manufacturer to manufacturer and from product to product. This page provides information on techniques used to determine pore size, what calculations are used, and what functional consequences pore size may have with respect to the intended application of the membrane filter.
The membranes ability to retain particular molecules of interest is based on the filters retentiveness characteristics. Retentiveness is sometimes related to pore size, but is also a function of the particular application, sample matrix, solvent and filtration conditions.
Migration and invasion assay chart provides you with some information about the pore sizes, plate formats, ECM Coating , detection, number of tests and catalogue number of all our Migration & Invasion Assays.
ProSep%C2%AE-vA Ultra chromatography resin provides high binding capacity and improved process economics for the capture and purification of monoclonal, polyclonal and engineered antibodies. The pore size of its controlled pore glass base matrix is smaller providing significantly more surface area which, when derivitized at the same protein A surface density, results in a significant increase in IgG binding capacity.
Flow refers to the time it takes for a particular flow stream to pass through the filter. The flow rate of a filter is important in determining how rapidly filtration can be completed. This page describes how membrane and solute characteristics both affect flow rate. Although flow rate generally decreases with pore size among membranes of a single type, membranes with the same pore size rating, but made from different materials or by different methods, can have very different flow rates. Flow rate differences can be caused by differences in thickness, porosity, and pore architecture.
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.