The production of antibodies and Fc-fusion proteins involves several downstream processing unit operations. The widely used purification template with Protein A chromatography, virus inactivation at low pH, and subsequent ion exchange chromatography steps is mostly able to remove impurities like aggregates, host-cell proteins, and viruses, which could affect the safety and efficacy of the product.
The low pH elution during Protein A chromatography, as well as during virus inactivation may induce aggregation. Preventing protein aggregation during these unit operations instead of removing the multimeric forms during subsequent polishing steps would be a more efficient strategy. Curious to learn more how the aggregation levels in the final product formulation can be minimized by excipients? Click on below image to check out our new poster “Application of Excipients in Downstream Processing”.
|Sugars & Polyols||Amino Acids|
Sucrose EMPROVE® EXPERT
L-Arginine monohydrochloride EMPROVE® EXPERT
Parteck® SI 400 LEX (Sorbitol) suitable for use as excipient EMPROVE® exp
L-Arginine EMPROVE® EXPERT
D(-)-Mannitol EMPROVE® EXPERT
Glycine cryst. EMPROVE® EXPERT
Trehalose dihydrate EMPROVE® EXPERT
Glycine granulated EMPROVE® EXPERT
Glycerol EMPROVE® EXPERT
L-Histidine EMPROVE® EXPERT
L-Proline EMPROVE® EXPERT
L-Histidine monohydrochloride monohydrate EMPROVE® EXPERT