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LiChrospher® RP-Select B (5 µm) HPLC Columns

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ChromolithMilliporeSigma offers a broad range of HPLC columns that are fully compliant with USP (United States Pharmacopoeia) specifications. L7 USP columns are described as octyl silane (C8), chemically linked to porous silica particles, 1.5 to 10 µm in diameter. Our high-quality L7 USP columns include: LiChrospher® RP-Select B (5 µm).

Excellent Separations of Basic Compounds

LiChrospher® 60 RP-Select B (5 µm) is a versatile reversed-phase sorbent, optimized for HPLC separations of basic compounds. Based on spherical silica particles, the sorbent prevents secondary interactions with basic substances, and ensures their elution as highly symmetrical peaks. LiChrospher® 60 RP-Select B is also suitable for the determination of neutral and acidic substances.

Master Batch Production for Maximum Reliability

Several individual batches of LiChrospher® RP-select B are used to produce a large master batch. This ensures the sorbent’s excellent batch-to-batch consistency. Consequently, LiChrospher® RP-select B delivers highly reproducible results, and secures the reliability of your HPLC method.

Tanaka 1 Test

The superior reproducibility of the “master batch LiChrospher® RP-select B" compared to small individual batches of the sorbent is demonstrated with the Tanaka test.

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Features and Benefits

  • Classified as L7 USP column
  • Optimal HPLC separations of basic compounds
  • Symmetrical peak shapes
  • No secondary interactions with basic substances
  • Master batch concept ensures highly reproducible results
  • Also suitable for neutral and acidic substances

Application Examples

Environmental analysis: Phenols

Environmental analysis: Fungicides in wine

Separation of alkaloids

Specification of LiChrospher® RP-Select B (5 µm)

Sorbent characteristics Particles of silica with octadecyl derivative
Particle shape Spherical
Particle size 5 μm
Pore size 60 Å (6 nm)
Pore volume 0.9 mL/g
Surface area 360 m2/g
Carbon load 11.5 % C
Coverage of the surface 3.55 μmol/m2
Efficiency 55,000 N/m
pH range 2-7.5
Shipping eluent Acetonitrile/water
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