Millipore Sigma Vibrant Logo

L1 US Pharmacopoeia Columns

Request Information

Purospher® RP-18 endcapped (5 µm) HPLC Columns

Purospher® RP-18 endcapped HPLC columnMilliporeSigma offers a broad range of HPLC columns that are fully compliant with USP (United States Pharmacopoeia) specifications. L1 USP columns are described as octadecyl silane, chemically linked to porous silica or ceramic micro particles. Our high-quality L1 USP products deliver reliable, reproducible results – time after time. A perfect example is: Purospher® RP-18 endcapped (5 µm).

Features and Benefits

  • Classified as L1 USP column
  • Excellent selectivity for both basic and strongly acidic compounds
  • Robust and fast method development with simple, neutral eluents
  • Suitable for the determination of azo dyestuff amines and beta-blockers

One Column for Both Basic and Strongly Acidic Compounds

Purospher® RP-18 endcapped is a versatile HPLC column that delivers separations with excellent peak symmetry for both basic and strongly acidic compounds. The column’s exceptional balance of chromatographic properties allows successful separations of complex samples, such as drugs, with simple, neutral eluents. This reduces analysis time while increasing lab productivity. Thanks to its optimal selectivity and efficiency, Purospher® RP-18 endcapped allows robust method development in R&D and QC.

Application Example

Purity, Peak Symmetry and Stability

Purospher® RP-18 endcapped columns are based on high-purity, metal-free silica with virtually complete surface coverage. This enables the elution of acidic, basic, and chelating compounds with no peak tailing. The columns are particularly suitable for the determination of various azo dyestuff amines and beta-blockers, demonstrating high resolution and peak symmetry. Thanks to their chemical stability, Purospher® RP-18 endcapped columns can easily tolerate mobile phase conditions at pH 8 for a considerably long time without loss of performance.

Application Example


Sorbent characteristics
High-purity C18-silica with special modification and deactivation of the surface
Metal content
Na, Ca, Mg, Al: 1 ppm; Fe: 3 ppm
Particle shape
Particle size
5 μm
Pore size 90 Å (9 nm)
Pore volume 1.05 mL/g
Spec. surface area 480 m²/g
Carbon load 18.0 % C
pH range pH 2 - 8
Efficiency 80.000 N/m
Shipping eluent Acetonitrile/Water
Application Notes Finder
Search for chromatography applications by analyte, column type, detection method, sorbent type or any other search term:
Chromatography Application World

Application World

The ultimate source for the latest news in your field

Learn More
Education, support, and events

Education, Support & Events: All the resources you need to succeed.

Learn More