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71339 pIEx™-7 Ek/LIC Vector Kit - Novagen

71339
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      Description
      OverviewThe pIEx™-7 Ek/LIC Vector is a ligation-independent cloning-prepared derivative of the pIEx-6 vector designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells and a minimal N-terminal His•Tag® coding sequence. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. The Ek/LIC cloning site is designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection and quantification (1).
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number71339
      Brand Family Novagen®
      SynonymsInsectDirect™ pIEx™-7 Ek/LIC Vector Kit
      References
      ReferencesKim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.
      Product Information
      1 µgEk/LIC Vector
      25 unitsT4 DNA Polymerase, LIC-qualified
      50 µl25 mM EDTA
      40 µl25 mM dATP
      8 µlEk/LIC β-gal Control Insert
      100 µl100 mM DTT
      50 µl10X T4 DNA Polymerase Buffer
      1.5 mlNuclease-free Water
      5 × 2 mlSOC Medium
      10 µlTest Plasmid
      22 × 50 µlNovaBlue GigaSingles™ Competent Cells
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pIEx™-7 Ek/LIC Vector Kit - Novagen Certificates of Analysis

      TitleLot Number
      71339

      References

      Reference overview
      Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.

      Brochure

      Title
      Radiance!" Multisystem Protein Expression

      Citations

      Title
    • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189-194.
    • User Protocols

      Title
      TB163 Ek/LIC Cloning Kits

      Vector Map

      Title
      TB399VM pIEx-7 Ek/LIC Vector Map

      Vector Sequence

      Title
      pIEx-7 Ek/LIC Vector Sequence