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71148 pETcoco™-2 DNA - Novagen

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      Plastic ampoule 10 μg
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      OverviewThe pETcoco™-2 expression vector is designed to allow "on command" amplification of vector copy number from single copy (1–2 plasmid copies per cell) to medium copy (20–50 per cell). The vector is maintained in the single copy state by utiliziing the oriS and repE elements of the F episome together with the parABC partition determinants. The medium copy state is obtained by employing the RK-2 derived trfA replicator acting at oriV. The switch from single copy to medium copy status is achieved by inducing the expression of the trfA gene with arabinose. The dual replicon nature of these vectors promotes enhanced stability of recombinant plasmids and extremely low basal expression levels in the single copy state, while allowing robust target gene expression typical of the pET vectors. pETcoco-2 contains the ampicillin resistance gene. The sequence is numbered relative to the start of T7 transcription at +1.

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.

      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to:
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number71148
      Brand Family Novagen®
      Product Information
      Fusion tagHis•Tag, S•Tag, HSV•Tag
      Vector familypETcoco
      Quality LevelMQ100
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalog Number GTIN
      71148-3 04055977254815


      pETcoco™-2 DNA - Novagen SDS


      Safety Data Sheet (SDS) 

      pETcoco™-2 DNA - Novagen Certificates of Analysis

      TitleLot Number


    • Adam Robertson, Steven R. Pattishall and Steven W. Matson. (2006) The DNA binding activity of mutL Is required for methyl-directed mismatch repair in Escherichia coli. Journal of Biological Chemistry 281, 8399-8408.
    • An X. Tran, et al. (2004) Periplasmic cleavage and modification of the 1-phosphate group of Helicobacter pylori lipid A. Journal of Biological Chemistry 279, 55780-55791.
    • User Protocols

      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB333 pETcoco™ System

      Vector Map

      TB335VM pETcoco™-2 Vector Map

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