71131 pETcoco™-1 System - Novagen

71131
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
71131-3
Retrieving availability...
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Plastic ampoule 1 kit
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity needs to be mulitiple of
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      Description
      OverviewThe pETcoco™ System combines the advantages of T7 promoter-driven protein expression with the ability to control plasmid copy number (Sektas 2003). In the presence of 0.2% glucose, pETcoco™ recombinant plasmids are maintained at one copy per cell. In this single-copy state, pETcoco clones are extremely stable because background expression and consequent gene rearrangements or deletions are virtually absent. For plasmid preparation, the copy number can be amplified to approximately 40 copies per cell independently of target gene expression by addition of L-arabinose to the culture medium. Both target gene expression and plasmid plus insert amplification are induced upon addition of IPTG. Both pETcoco-1 and pETcoco-2 vectors feature an N-terminal His•Tag and internal S•Tag™ sequences, both of which are cleavable by enterokinase, and an optional C-terminal HSV•Tag sequence. NovaF- Competent Cells for cloning target genes and Tuner™(DE3) Competent Cells for expression are supplied with the pETcoco-1 and pETcoco-2 Systems.



      Coexpression
      The pETcoco-1 plasmid contains a pET vector-compatible origin of replication and chloramphenicol resistance marker, making it suitable for transformation into cells already containing ampicillin- or kanamycin-resistant pET or pETDuet™-1 recombinants to enable the coexpression of multiple target genes in the same cell. The ampicillin-resistant pETcoco-2 plasmid is compatible with pET host strains supplying rare codon tRNAs and/or T7 lysozyme on a chloramphenicol-resistant plasmid, and with kanamycin-resistant pET recombinants for coexpression purposes. pETcoco-2 may also be used for coexpression with pACYCDuet™-1 recombinants with appropriate host strains.






      Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number71131
      Brand Family Novagen®
      Features and benefits
      • T7 promoter-driven protein expression
      • Offers the ability to control plasmid copy number
      • Works with toxic proteins
      • N-terminal His•Tag® sequence
      • Internal S•Tag™ sequence
      • Optional C-termainal HSV•Tag® sequence
      References
      References

      Sektas, M. and Szybalski, W. 2003. inNovations 14, 6.

      Product Information
      Components
      Fusion tagHis•Tag, S•Tag, HSV•Tag
      Vector familypETcoco
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pETcoco™-1 System - Novagen SDS

      Title

      Safety Data Sheet (SDS) 

      pETcoco™-1 System - Novagen Certificates of Analysis

      TitleLot Number
      71131

      References

      Reference overview

      Sektas, M. and Szybalski, W. 2003. inNovations 14, 6.

      Citations

      Title
    • Rachel Balder, et al. (2007) Moraxella catarrhalis strain O35E expresses two filamentous hemagglutinin-like proteins that mediate adherence to human epithelial cells. Infection and Immunity 75, 2765-2775.
    • User Protocols

      Title
      TB333 pETcoco™ System

      Vector Map

      Title
      TB331VM pETcoco™-1 Vector Map

      Related Products & Applications

      Related Products

      Catalog Number Description  
      71149 pETcoco™-2 System - Novagen Show Pricing & Availability

      Related Products By: Application Facete

      Related Products By: Brand Facete

      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors