69863 pET-27b(+) DNA - Novagen

69863
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pET-27b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal HSV•Tag® and His•Tag® sequences. This vector differs from pET-25b(+) only by its selectable marker (kanamycin vs. ampicillin resistance). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB073).

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69863
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
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      Toxicological Information
      Safety Information according to GHS
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      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
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      Documentation

      pET-27b(+) DNA - Novagen Certificates of Analysis

      TitleLot Number
      69863

      Citations

      Title
    • Jason Cellitti, et al. (2007) Exploring subdomain cooperativity in T4 lysozyme I: Structural and energetic studies of a circular permutant and protein fragment. Protein Science 16, 842-851.
    • Asa Flanigan and Jeffrey F. Gardner. (2007) Interaction of the gifsy-1 xis protein with the gifsy-1 attP sequence. Journal of Bacteriology 189, 6303-6311.
    • R. Jeremy Johnson, Shawn R. Lin and Ronald T. Raines. (2007) Genetic selection reveals the role of a buried, conserved polar residue. Protein Science 16, 1609-1616.
    • Danxu Liu, Paul Crellin and Ronald Chalmers. (2005) Cyclic changes in the affinity of protein-DNA interactions drive the progression and regulate the outcome of the Tn10 transposition reaction. Nucleic Acids Research 33, 1982-1992.
    • Ken-Shwo Dai and Choong-Chin Liew. (2001) A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via Its RING domain. Journal of Biological Chemistry 276, 23992-23999.
    • Jeffrey J. Brandt, Lisa L. Chatwood and Michael W. Crowder. (2000) Analysis of three overexpression systems for VanX, the zinc(II) dipeptidase required for high-level vancomycin resistance in bacteria. Protein Expression and Purification 20, 300-307.
    • B. Kenny, et al. (1997) Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into ammmalian cells. Cell 91, 511-520.
    • User Protocols

      Title
      TB055 pET System Manual

      Vector Map

      Title
      TB073VM pET-27b(+) Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors