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69739 pET-20b(+) DNA - Novagen

69739
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      Plastic ampoule 10 μg
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      Description
      OverviewThe pET-20b(+) vector carries an N-terminal pelB signal sequence for potential periplasmic localization, plus optional C-terminal His•Tag® sequence. Unique sites are shown on the vector map; note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB037).

      The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      Catalogue Number69739
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      pET-20b(+) DNA - Novagen Certificates of Analysis

      TitleLot Number
      69739

      Citations

      Title
    • Gillian A. Owen, et al. (2007) Zinc-responsive regulation of alternative ribosomal protein genes in Streptomyces coelicolor involves Zur and ςR. Journal of Bacteriology 189, 4078-4086.
    • Johanna Kolln, et al. (2006) Triosephosphate isomerase- and glyceraldehyde-3-phosphate dehydrogenase-reactive autoantibodies in the cerebrospinal fluid of patients with multiple sclerosis. Journal of Immunology 177, 5652-5658.
    • Chaitali Basak, et al. (2005) The secreted peptidyl prolyl cis,trans-isomerase HP0175 of Helicobacter pylori induces apoptosis of gastric epithelial cells in a TLR4- and apoptosis signal-regulating kinase 1-dependent manner. Journal of Immunology 174, 5672-5680.
    • Hsiu-Hui Chen, et al. (2005) Fusion proteins comprising annexin V and Kunitz protease inhibitors are highly potent thrombogenic site-directed anticoagulants. Blood 105, 3902-3909.
    • Melissa L. Geddie, et al. (2005) Rational design of p53, an intrinsically unstructured protein, for the fabrication of novel molecular sensors. Journal of Biological Chemistry 280, 35641-35646.
    • Agnes E. Hamburger, et al. (2005) Steric accessibility of the HIV-1 gp41 N-trimer region. Journal of Biological Chemistry 280, 12567-12572.
    • Eiko Nakamaru-Ogiso, et al. (2005) Characterization of the iron-sulfur cluster N7 (N1c) in the subunit nuoG of the proton-translocating NADH-quinone oxidoreductase from Escherichia coli. Journal of Biological Chemistry 280, 301-307.
    • Baohai Shao, et al. (2005) Tyrosine 192 in apolipoprotein A-I is the major site of nitration and chlorination by myeloperoxidase, but only chlorination markedly impairs ABCA1-dependent cholesterol transport. Journal of Biological Chemistry 280, 5983-5993.
    • Dhammika Gunasekera and Robert G. Kemp. (1999) Cloning, sequencing, expression, and purification of the C isozyme of mouse phosphofructokinase. Protein Expression and Purification 16, 448-453.
    • User Protocols

      Title
      TB055 pET System Manual

      Vector Map

      Title
      TB037VM pET-20b(+) Vector Map

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      Categories

      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors