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|71340-3||Plastic ampoule||10 μg||
|Overview||pCDFDuet™-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet™-1, pACYCDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.
This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: firstname.lastname@example.org.
LaneM: Trail Mix™ Protein Markers; Lane1: pACYDuet-1:β-gal + Fluc; Lane2: pETDuet-1:GST/GUS + GFP; Lane3: pRSFDuet-1:Nus/hlFNγ + S•Tag/T4 PNK; Lane4: pCDFDuet-1:GUS + His•Tag/MBP; Lane5: pACYDuet-1, pETDuet-1 combination; Lane6: pACYDuet-1, pRSFDuet-1 combination; Lane7: pACYDuet-1, pCDFDuet-1 combination; Lane8: pETDuet-1, pRSFDuet-1 combination; Lane9: pRSFDuet-1, pCDFDuet-1 combination; Lane10: pETDuet-1, pCDFDuet-1 combination; Lane11: pACYDuet-1, pETDuet-1, pRSFDuet-1 combination; Lane12: pACYDuet-1, pETDuet-1, pCDFDuet-1 combination; Lane13: pACYDuet-1, pRSFDuet-1, pCDFDuet-1 combination; Lane14: pETDuet-1, pRSFDuet-1, pCDFDuet-1 combination; Lane15: Uninduced control; Lane16: All four Duet vectors: all eight proteins
The indicated constructs were transformed individually or together into BL21(DE3). Cultures were grown in TB plus phosphates plus glucose at 37°C to and Abs600 between 1.0 and 1.2. Target protein expression was induced by adding IPTG to a final concentration of 1 mM. Cultures were harvested by centrifugation 2.5 h after induction. Lysates were produced by sonication using equal volumes of 1% SDS and 2X SDS sample buffer. Equivalent amounts of protein (based on harvest ABS) were analyzed by SDS-PAGE (4-20% gradient gel) and stained with coomassie blue. To maximize band separation, proteins smaller than 25 kDa were allowed to migrate off the gel.
|Safety Information according to GHS|
|Product Usage Statements|
|Storage and Shipping Information|
|Ship Code||Shipped with Blue Ice or with Dry Ice|
|Do not freeze||Ok to freeze|
pCDFDuet™-1 DNA - Novagen SDS
|TB340 Duet Vectors|
|TB401 pRSF and pCDF Vectors|
|TB390VM pCDFDuet™-1 Vector Map|
|pCDFDuet™-1 Vector Sequence|