532980 miR-34a Activator, Rubone - Calbiochem

532980
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₂₀H₂₂O₇

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      Glass bottle 10 mg
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      Description
      OverviewA cell-permeable chalcone derivative that preferentially increases primary and mature miR-34a levels in Huh7 and HepG2 hepatocellular carcinoma cells (HCC) expressing wild-type or mutated p53, but does not affect miR-34a expression in non-tumorigenic Hep3B cells with deleted p53. Inhibits the luciferase activity in a HCC cell-based miR34a luciferase reporter system (IC50 = 3.8 µM). Causes a significant reduction in the mRNA and protein levels of cyclin D1 and Bcl-2 in Huh7 and HepG2 cells (~ 10 µM), but not in Hep3B cells. Also shown to reduce the levels of miR-34a targets (Cdk6, FOXP, Notch 1 and SIRT) and p53 targets (p21 and PUMA) in Huh7 and HepG2 cells, but not in Hep3B cells. Inhibits the growth of multiple HCC by inducing apoptosis and reducing proliferation, but shows no toxicity on non-tumorigenic human hepatocytes. Suppresses the growth of Huh7 and HepG2 xenografts in nude mice model (~90% inhibition at 50 mg/kg).

      Please note that the molecular weight for this compound is batch-specific due to variable water content.
      Catalogue Number532980
      Brand Family Calbiochem®
      SynonymsmiroRNA-34a Activator, Rubone
      References
      ReferencesXiao, Z., et al. 2014. Cancer Res. 74, 6236.
      Product Information
      FormYellow powder
      Hill FormulaC₂₀H₂₂O₇
      Chemical formulaC₂₀H₂₂O₇
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetmiR-34a
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      miR-34a Activator, Rubone - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Xiao, Z., et al. 2014. Cancer Res. 74, 6236.

      Technical Info

      Title
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      White Paper: Further considerations of antibody validation and usage. (EMD)
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-March-2015 JSW
      SynonymsmiroRNA-34a Activator, Rubone
      DescriptionA cell-permeable chalcone derivative that preferentially increases primary and mature miR-34a levels in Huh7 and HepG2 hepatocellular carcinoma cells (HCC) expressing wild-type or mutated p53, but does not affect miR-34a expression in non-tumorigenic Hep3B cells with deleted p53. Inhibits the luciferase activity in a HCC cell-based miR34a luciferase reporter system (IC50 = 3.8 µM). Causes a significant reduction in the mRNA and protein levels of cyclin D1 and Bcl-2 in Huh7 and HepG2 cells (~ 10 µM), but not in Hep3B cells. Also shown to reduce the levels of miR-34a targets (Cdk6, FOXP, Notch 1 and SIRT) and p53 targets (p21 and PUMA) in Huh7 and HepG2 cells, but not in Hep3B cells. Inhibits the growth of multiple HCC by inducing apoptosis and reducing proliferation, but shows no toxicity on non-tumorigenic human hepatocytes. Suppresses the growth of Huh7 and HepG2 xenografts in nude mice model (~90% inhibition at 50 mg/kg).
      FormYellow powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₀H₂₂O₇
      Purity≥98% by HPLC
      SolubilityDMSO (10 mg/ml)
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesXiao, Z., et al. 2014. Cancer Res. 74, 6236.