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500554 mTOR Activator, MHY1485 - Calbiochem

500554
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₁₇H₂₁N₇O₄

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      Glass bottle 10 mg
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      Description
      OverviewA cell-permeable triazine derivative that is shown to increase cellular mTOR Ser2448 and downstream substrate 4E-BP Thr37/46 phosphorylation level in rat liver Ac2F cells (1 to 2 µM for 1 h), affecting culture viability only at much higher concentrations (by 20% after 24 h 20 µM treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes. The mechanism of mTOR activation by MHY1485 is currently unknown.
      Catalogue Number500554
      Brand Family Calbiochem®
      Synonyms4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine, Autophagy Inhibitor III
      References
      ReferencesChoi, Y. J., et al. 2012. PLoS. One. 7, e43418.
      Product Information
      FormPale yellow solid
      Hill FormulaC₁₇H₂₁N₇O₄
      Chemical formulaC₁₇H₂₁N₇O₄
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      ApplicationmTOR Activator, MHY1485, CAS Number: 326914-06-1, is a cell-permeable activator of mTOR that inhibits autophagy by suppressing the fusion between autophagosomes and lysosomes.
      Biological Information
      Primary Target IC<sub>50</sub>~2 µ
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      mTOR Activator, MHY1485 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Choi, Y. J., et al. 2012. PLoS. One. 7, e43418.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-May-2013 JSW
      Synonyms4,6-dimorpholino-N-(4-nitrophenyl)-1,3,5-triazin-2-amine, Autophagy Inhibitor III
      DescriptionA cell-permeable triazine derivative that is shown to increase cellular phosphorylation level of mTOR Ser2448 (20% and 42% above basal in 1 h, respectively, with 1 and 2 µM MHY1485) and downstream substrate 4E-BP Thr37/46 (29% and 34% above basal in 1 h, respectively, with 1 and 2 µM MHY1485) in rat liver Ac2F cells, affecting culture viability only at much higher concentrations (by 20% after 24 h 20 µM treatment). MHY1485 induced cellular LC3-II accumulation in Ac2F (117%, 150%, and 250% of vehicle control LC3-II/LC3-I ratio, respectively, with 0.5, 1, and 2 µM treatment for 6 h; 142% and 172% of control ratio, respectively, with 2 µM treatment for 1 h or 12 h) is reported to be a result of autophagy inhibition due to reduced fusion between autophagosomes and lysosomes as demonstrated by a reduction of starvation-induced AdGFP-LC3 and lysosome dye co-localization (27% and 32.7% above control co-localization in 6 h serum deprived cultures with or without 2 µM MHY1485; 21% above control co-localization with 2 µM MHY1485 treatment alone and without serum starvation). The mechanism of mTOR activation by MHY1485 is currently unknown.
      FormPale yellow solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₇H₂₁N₇O₄
      Structure formulaStructure formula
      Purity≥97% by HPLC
      SolubilityDMSO (12.5 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesChoi, Y. J., et al. 2012. PLoS. One. 7, e43418.