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ZipTip® Pipette Tips

Concentrating and purifying samples for MALDI-ToF MS

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Overview

Specifications

Ordering Information

ZipTip® Pipette TipsClear Sorting & Filtering Show Filter
Catalog NumberDescriptionPack Size
ZTC18S008ZipTip with 0.6 µL C18 resin 8 Show Pricing & Availability
ZTC18S096ZipTip with 0.6 µL C18 resin 96 Show Pricing & Availability
ZTC18S960ZipTip with 0.6 µL C18 resin 960 Show Pricing & Availability
ZTC04S008ZipTip with 0.6 µL C4 resin 8 Show Pricing & Availability
ZTC04S096ZipTip with 0.6 µL C4 resin 96 Show Pricing & Availability
ZTC04S960ZipTip with 0.6 µL C4 resin 960 Show Pricing & Availability
ZTC18M008ZipTip with 0.2 µL C18 resin 8 Show Pricing & Availability
ZTC18M096ZipTip with 0.2 µL C18 resin 96 Show Pricing & Availability
ZTC18M960ZipTip with 0.2 µL C18 resin 960 Show Pricing & Availability
ZTSCXS008ZipTip with 0.6 µL strong cation resin 8 Show Pricing & Availability
ZTSCXS096ZipTip with 0.6 µL strong cation resin 96 Show Pricing & Availability

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Documentation

Technical Info

Title
Purification of Radiolabeled Oligonucleotides Using ZipTipC18 Pipette Tips

Data Sheet

Title
Mass Spectrometry Workflow Solutions

Posters

Title
Enhanced MALDI-TOF MS Spectra of Serum Peptides Using Ultrafiltration ; ASMS 2002
Poster: Purification of Peptides from Serum and Cell Lysates for Mass Spectrometry, ASMS, 2004
Zip....Tips: Creative Ways to Use ZipTip Pipette Tips (ABRF 2003)
ZipTip Chemistries Poster: Micro-Adsorptive Sample Preparation for Mass Spectrometry

References

Reference overviewApplicationPub Med ID
FOOD PEPTIDOMICS OF IN VITRO GASTROINTESTINAL DIGESTIONS OF PARTIALLY PURIFIED BOVINE HEMOGLOBIN: LOW-RESOLUTION VERSUS HIGH-RESOLUTION LC-MS/MS ANALYSES
Juliette Caron, Gabrielle Chataigne, Jean-Pascal Gimeno, Nathalie Duhal, Jean-Francois Goossens, Pascal Dhulster, Benoit Cudennec, Rozenn Ravallec, Christophe Flahaut
Electrophoresis  2016

Show Abstract
26990205 26990205
A CROSS-SPECIES STUDY OF PI3K PROTEIN-PROTEIN INTERACTIONS REVEALS THE DIRECT INTERACTION OF P85 AND SHP2
Susanne B. Breitkopf, Xuemei Yang, Michael J. Begley, Meghana Kulkarni, Yu-Hsin Chiu, Alexa B. Turke, Jessica Lauriol, Min Yuan, Jie Qi, Jeffrey A. Engelman, Pengyu Hong, Maria I. Kontaridis, Lewis C. Cantley, Norbert Perrimon, John M. Asara
Sci Rep.  2016

Show Abstract
26839216 26839216
Comparative evaluation of peptide desalting methods for salivary proteome analysis.
Nico Jehmlich, Claas Golatowski, Annette Murr, Gesell Salazar, Vishnu Mukund Dhople, Elke Hammer, Uwe Volker
Clin Chim Acta  2014

24751662 24751662
ISOLATION AND IDENTIFICATION OF MEMBRANE VESICLE-ASSOCIATED PROTEINS IN GRAM-POSITIVE BACTERIA AND MYCOBACTERIA
Rafael Prados-Rosales, Lisa Brown, Arturo Casadevall, Sandra Montalvo-Quiros, Jose L. Luque-Garcia
MethodsX.  2014

Show Abstract
26150943 26150943
Using theoretical protein isotopic distributions to parse small-mass-difference post-translational modifications via mass spectrometry.
Timothy W. Rhoads, Jared R. Williams, Nathan I. Lopez, Jeffrey T. Morre, C. Samuel Bradford, Joseph S. Beckman
J Am Soc Mass Spectrom  2013

23247967 23247967
DETERMINIG IN VIVO PHOSPHORYLATION SITES USING MASS SPECTROMETRY
Susanne B. Breitkopf, John M. Asara
Curr Protoc Mol Biol  2012

Show Abstract
22470061 22470061
Rapid metabolite identification with sub parts-per-million mass accuracy from biological matrices by direct infusion nanoelectrospray ionization after clean-up on a ZipTip and LTQ/Orbitrap mass spectrometry.
John C. L. Erve, William DeMaio, Rasmy E. Talaat
Rapid Commun Mass Spectrom  2008

Show Abstract
18763271 18763271
Analysis of modified apolipoprotein B-100 structures formed in oxidized low-density lipoprotein using LC-MS/MS.
Takashi Obama, Rina Kato, Yutaka Masuda, Katsuhiko Takahashi, Toshihiro Aiuchi, Hiroyuki Itabe
Proteomics  2007

Show Abstract
17549798 17549798
GEL-FREE SAMPLE PREPARATION FOR THE NANOSCALE LC-MS/MS ANALYSIS AND IDENTIFICATION OF LOW-NANOGRAM PROTEIN SAMPLES
Marco Gaspari, Vittorio Abbonante, Giovanni Cuda
J Sep Sci.  2007

Show Abstract
17763504 17763504
COMPARISON OF SDS- AND METHANOL-ASSISTED PROTEIN SOLUBILIZATION AND DIGESTION METHODS FOR ESCHERICHIA COLI MEMBRANE PROTEOME ANALYSIS BY 2-D LC-MS/MS
Nan Zhang, Rui Chen, Nelson Young, David Wishart, Philip Winter, Joel H. Weiner, Liang Li
Proteomics  2007

17309111 17309111

FAQ

QuestionAnswer
What are the specifications of the SCX resin in the ZipTipSCX Pipette Tips?SCX resin is 12 mm beads with a 300 A pore size.
What is the binding capacity of ZipTipSCX?Binding capacity of ZipTipSCX is about 200-250 ng.
The mass spectra results indicate loss of peptides after purifying my sample with ZipTip-SCX. What do you suggest to improve my recoveries?We recommend pre-washing the tips with the elution solution (5% NH4OH, 30% MeOH) priop to equilibration with washing solution (0.1% TFA). This improves binding.

In addition, peptides should be bound under acidic conditions and eluted under basic conditions. It is recommended that you check the pH of your binding colution with pH paper to ensure it is acidic.

It may be that the eluted peptides were not allowed to dry completely before the matrix was added to the MALDI plate.
Can I use ZipTipSCX to purify samples from solutions containing salts?Yes, we have seen efficient binding from solutions containing up to 0.5M NaCl under acdic conditions. If the salt solution is basic binding will not occur.
When is it recommended to use ZipTipC18 versus ZipTip SCX?For all applications involving the desalting and concentration of peptides and proteins from aqueous solutions we recommend the ZipTipC18. If the peptide solutions caontains solvent or detergent we recommend the ZipTipSCX.
Can I use ZipTip SCX to remove glycerol from my sample?Gylcerol will interfere with mass spectroscopy. ZipTipSCX can remove up to 20-30% glycerol from a sample.
My spots on the MALDI plate are yellow after purifying my sample with ZipTip SCX. What could this be due to?This yellow color is due to the peptide sample was not fully dried on the MALDI plate before the matrix was applied. It is important for the sample to dry completely before the matrix is applied or you may not see any signal.
Can I use higher than 50% ACN to elute my sample from ZipTipC18 and ZipTipm-C18?Yes, however the tip should be prewet 5 times with 10 ml of 100% ACN followed by a wash of 10 ml 0.1% TFA. Then bind, wash and elute.
After elution from ZipTip my spots won't dry. Want can I do?For the initial prewet step wash the tip 5 times with 100% ACN followed by 0.1% TFA. Then bind, wash and elute as normal.
Are ZipTip pipette tips compatible with robotic / automated sample preparation systems?ZipTipm-C18 compatibility has been demonstarted with the following robotic / automated sample preparation systems:

1. ABI Symbiot™ Sample Workstation for MALDI TOF Biospectrometry™ with Universal Cone for ZipTip (cat. no. GEN602987) and Rack Adapter for the ZipTip box.

2. Bruker Daltonics MAP™ II and MAP™ II/8 MADLI Auto Prep Station

3. Micromass 2700-MS and MassPREP™ Sample Preparation Stations

4. Genomic Solutions ProMS™ Sample Preparation Station

5. Cy-Bio CyBi™ Well 96 automated pipettor

6. Packard BioScrences MultiPROBE

7. Tecan GENESIS Workstation

User Guides

Title
ZipTip SCX Pipette Tips