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71186 YeastBuster™ Protein Extraction Reagent

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71186
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71186-3
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      Plastic ampoule 100 ml
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      71186-4
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          Plastic ampoule 500 ml
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          Description
          OverviewYeastBuster™ Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.

          In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind™ and Ni-NTA His•Bind® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.








          Catalogue Number71186
          Brand Family Novagen®
          Features and benefits
          • Gentle, rapid, efficient extraction of proteins from yeast cells
          • Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
          • Higher yield of total and enzymatically active proteins
          • Fully compatible with Ni-NTA His•Bind® and GST•Bind™ affinity purification methods
          References
          Product Information
          Components
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage Multiple storage requirements
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          YeastBuster™ Protein Extraction Reagent SDS

          Title

          Safety Data Sheet (SDS) 

          YeastBuster™ Protein Extraction Reagent Certificates of Analysis

          TitleLot Number
          71186

          Brochure

          Title
          Protein Purification and Detection Tools
          The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression (MilliporeSigma)

          Technical Info

          Title
          TB316 YeastBuster Protein Extraction Reagent

          Citations

          Title
        • Jennifer A. Gibbons, et al. (2007) Expression of human protein phosphatase-1 in Saccharomyces cerevisiae highlights the role of phosphatase isoforms in regulating eukaryotic functions. Journal of Biological Chemistry 282, 21838-21847.
        • Alyssa Dilla, et al. (2004) The Arabidopsis F-Box protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation. The Plant Cell 16, 1392-1405.
        • User Protocols

          Title
          TB316 YeastBuster™ Extraction Reagent