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23-044 VHL Protein Complex, Active, 10 µg

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23-044
10 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpeciesCascade Component
      Enzyme AssaysHuman E3
      Description
      Catalogue Number23-044
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionVHL Protein Complex, Active, 10 µg
      Alternate Names
      • VCB-Cul2 complex
      • ECV complex
      • CBCVHL
      Background InformationThe VHL complex is a multi-subunit ubiquitin ligase composed of VHL, Elongin B, Elongin C, Cul2 and Rbx1. The VHL protein serves as the substrate recognition component and is linked by Elongin C to a heterodimeric Cul2/Rbx1 module that functions as a potent activator of the ubiquitination of target proteins by an E2 conjugating enzyme. Elongin B interacts with the complex through Elongin C and appears to stabilize the binding of Elongin C to VHL. The primary function of the VHL complex is to regulate HIF (hypoxia inducible factor) activity by targeting the hydroxylated HIF-1α subunit for ubiquitination and rapid proteasomal degradation under normoxic conditions. It therefore plays an important role in the regulation of hypoxia-inducible genes such as the vascular endothelial growth factor (VEGF) and glucose transport-1 (Glut-1). Mutations in VHL are associated with the inherited von Hippel-Lindau (VHL) cancer syndrome and numerous forms of renal cell carcinoma.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive complex of five (5) recombinant human enzymes: VHL amino acids 54-end, full length Elongin C, full length Elongin B, full length Cul2, & full length Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.
      Key Applications
      • Enzyme Assays
      Biological Information
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Cascade ComponentE3
      Entrez Gene Number
      Purification MethodPurified using glutathione sepharose.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Related Products & Applications

      Alternative Packsize

      Catalog Number Description  
      23-044M VHL Protein Complex, Active, 250 µg Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Proteins and Enzymes > Ubiquitin E3 Ligases
      Life Science Research > Drug Discovery and Development > Ubiquitin and Proteasome Screening > Ubiquitin E3 Ligases