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14-811 UbcH5c Conjugating Enzyme, 100 µg

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      Replacement Information

      Key Specifications Table

      Entrez Gene NumberSpeciesUni Prot Number
      NM_003340.4 NM_181886.1 NM_181887.1 NM_181888.1 NM_181889.1 NM_181890.1 NM_181891.1 NM_181892.1 NM_181893.1 Human P61077
      Catalogue Number14-811
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionUbcH5c Conjugating Enzyme, 100 µg
      OverviewThe His6-tagged fusion protein of UbcH5c is charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation. UbcH5c is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5c, together with UbcH5a and UbcH5b, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53, NF-κB, and c-Fos. Typical enzyme concentration to support conjugation in vitro is 100nM to 1μM depending upon conditions.
      Product Information
      Quality LevelMQ100
      ApplicationThe His6-tagged fusion protein of UbcH5c is charge & support ubiquitinylation in vitro. The His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.
      Biological Information
      Purity>95% by SDS-PAGE
      SourceRecombinant human UbcH5c Conjugating Enzyme expressed in E. coli.
      Entrez Gene Number
      Entrez Gene SummaryThe modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Multiple spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been determined.
      Gene Symbol
      • UBE2D3
      • UBCH5C
      • MGC43926
      • E2
      • UBC4/5
      • UbcH5C
      • E2(17)KB3
      • MGC5416
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P61077 # Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
      SIZE: 147 amino acids; 16687 Da
      SIMILARITY: SwissProt: P61077 ## Belongs to the ubiquitin-conjugating enzyme family.
      Molecular Weight17 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -80°C from date of shipment. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      UbcH5c Conjugating Enzyme, 100 µg SDS


      Safety Data Sheet (SDS) 

      UbcH5c Conjugating Enzyme, 100 µg Certificates of Analysis

      TitleLot Number
      UbcH5c Conjugating Enzyme (His6-tagged; human, recombinant protein) - 2141821 2141821
      UbcH5c Conjugating Enzyme (His6-tagged; human, recombinant protein) - 2449247 2449247
      UbcH5c Conjugating Enzyme (His6-tagged; human, recombinant protein) 3009993
      UbcH5c Conjugating Enzyme - 0611046119 0611046119
      UbcH5c Conjugating Enzyme - 2986004 2986004
      UbcH5c Conjugating Enzyme - 3540382 3540382


      Reference overviewPub Med ID
      Enzymes catalyzing ubiquitination and proteolytic processing of the p105 precursor of nuclear factor kappaB1
      Coux, O and Goldberg, A L
      J Biol Chem, 273:8820-8 (1998)  1998

      9535861 9535861
      Identification of a family of closely related human ubiquitin conjugating enzymes
      Jensen, J P, et al
      J Biol Chem, 270:30408-14 (1995)  1995

      8530467 8530467