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14-810 UbcH5b Conjugating Enzyme, 100 µg

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14-810
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Entrez Gene NumberSpeciesUni Prot Number
      NM_003339.2 NM_181838.1 Human P62837
      Description
      Catalogue Number14-810
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionUbcH5b Conjugating Enzyme, 100 µg
      OverviewThe His6-tagged fusion protein of UbcH5b is charge and support ubiquitinylation in vitro. Unlike their GST-tagged counterparts, the His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions. The His-tagged version of this enzyme is not susceptible to self-ubiquitinylation. UbcH5b is highly homologous to the S. cerevisiae UbC4/5 family of E2 enzymes, demonstrated to be essential for degradation of regulatory and abnormal proteins. UbcH5b, together with UbcH5a and UbcH5c, are the most active class of E2 enzymes in cell extracts and are associated with regulation of a number of transcription factors including p53, NF-κB, and c-Fos.
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationThe His6-tagged fusion protein of UbcH5b is charge & support ubiquitinylation in vitro. The His6-tagged UbcH5 family members all appear to form thiol ester conjugates with ubiquitin at a similar rate under similar conditions.
      Biological Information
      Purity>95% by SDS-PAGE
      SourceRecombinant human UbcH5b Conjugating Enzyme expressed in E. coli.
      Entrez Gene Number
      Entrez Gene SummaryThe modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
      Gene Symbol
      • UBE2D2
      • UBCH5B
      • PUBC1
      • E2
      • UbcH5B
      • UBC4/5
      • E2(17)KB2
      • UBC4
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62837 # Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53.
      SIZE: 147 amino acids; 16735 Da
      SUBUNIT: Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex.
      SIMILARITY: SwissProt: P62837 ## Belongs to the ubiquitin-conjugating enzyme family.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -80°C from date of shipment. Avoid multiple freeze/thaw cycles. After initial defrost, aliquot into individual tubes and refreeze at -80°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      UbcH5b Conjugating Enzyme, 100 µg SDS

      Title

      Safety Data Sheet (SDS) 

      UbcH5b Conjugating Enzyme, 100 µg Certificates of Analysis

      TitleLot Number
      UbcH5b Conjugating Enzyme (His6-tagged; human, recombinant protein) - 2381759 2381759
      UbcH5b Conjugating Enzyme (His6-tagged; human, recombinant protein) - 2421572 2421572
      UbcH5b Conjugating Enzyme (His6-tagged; human, recombinant protein) 3011363
      UbcH5b Conjugating Enzyme (His6-tagged; human, recombinant protein) 3085539
      UbcH5b Conjugating Enzyme (His6-tagged; human, recombinant protein) - 2020868 2020868
      UbcH5b Conjugating Enzyme (His6-tagged; human, recombinant protein) - 3407278 3407278
      UbcH5b Conjugating Enzyme (His6-tagged; human, recombinant protein) - 3707297 3707297
      UbcH5b Conjugating Enzyme - 0611046118 0611046118
      UbcH5b Conjugating Enzyme - 3170775 3170775
      UbcH5b Conjugating Enzyme - 3498486 3498486

      References

      Reference overviewPub Med ID
      Identification of the ubiquitin carrier proteins, E2s, involved in signal-induced conjugation and subsequent degradation of IkappaBalpha
      Gonen, H, et al
      J Biol Chem, 274:14823-30 (1999)  1999

      10329681 10329681
      Identification of a family of closely related human ubiquitin conjugating enzymes
      Jensen, J P, et al
      J Biol Chem, 270:30408-14 (1995)  1995

      8530467 8530467
      Ubiquitin-conjugating enzymes UBC4 and UBC5 mediate selective degradation of short-lived and abnormal proteins.
      Seufert, W and Jentsch, S
      EMBO J., 9: 543-50 (1990)  1990

      Show Abstract
      2154373 2154373