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UC0E03 UCOE® Dual Expression Puromycin Vector Set

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UC0E03
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      Overview

      Replacement Information
      Description
      Catalogue NumberUC0E03
      DescriptionUCOE® Dual Expression Puromycin Vector Set
      Alternate Names
      • UCOE Mammalian Protein Expression Vectors
      • UCOE Mammalian Protein Expression Plasmids
      • Ubiquitous Chromatin Opening Element
      • UCOE03
      Background InformationThe Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

      CET 1019 HD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.

      CET 1019 AD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.

      The CET 1019 HD-puro-SceI and CET 1019 AD-puro-SceI vectors possess the following features:

      • A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
      • A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3’ end formation
      • A puromycin antibiotic resistance gene for selection of stably transfected mammalian cells
      • A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
      • An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes

      References
      Antoniou, M., et al.. (2003) Transgenes Encompassing Dual Promoter CpG Islands from the Human TBP and HNRPA2B1 Loci are Resistant to Heterochromatin-mediated Silencing. Genomics, 82: 269-279.
      Antoniou, M., et al. (2010) A Ubiquitous Chromatin Opening Element (UCOE) Confers Resistance to DNA Methylation-mediated Silencing of Lentiviral Vectors. Mol Ther, 18: 1640-1649.

      THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.
      References
      Product Information
      Components
      • 1) 10ug CET 1019 HD-puro-SceI (+) Vector (CS221292)
      • 2) 10ug CET 1019 AD-puro-SceI (+) Vector (CS221293))
      • 3) 10ug DC HD-puro (-) Vector (CS221283)
      • 4) 10ug DC AD-puro (-) Vector (CS221295)
      Quality LevelMQ100
      Applications
      Application NotesUbiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression by removing epigenetic related gene silencing.
      Biological Information
      ConcentrationPlease refer to lot specific datasheet.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsPlasmids should be stored at -20C. Avoid repeated freeze-thaw events.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications