6502 Trypsin, Bovine Pancreas

View Products on Sigmaaldrich.com
6502
View Pricing & Availability

Overview

Replacement Information

Key Specifications Table

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
6502-2.5MU
Retrieving availability...
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Glass bottle 2.5 mu
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity needs to be mulitiple of
      Upon Order Completion More Information
      You Saved ()
       
      Request Pricing
      6502-25MU
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 25 mu
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewNative trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides and proteins at the C-terminal side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Note: 1 MU = 1,000,000 units.
          Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units.
          Catalogue Number6502
          Brand Family Calbiochem®
          References
          ReferencesBergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.
          Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem. 239, 1799.
          Hummel, B.C.W. 1955. Can. J. Biochem. Physiol. 37, 1393.
          Product Information
          CAS number9002-07-7
          Activity≥2,500 USP units/mg dry weight
          Unit of DefinitionOne USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
          EC number3.4.21.4
          FormSolid
          FormulationSalt-free
          Hygroscopic Hygroscopic
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          ContaminantsChymotrypsin: ≤50 NF units/mg dry weight
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSYN5075000
          Safety Information
          R PhraseR: 36/37/38-42

          Irritating to eyes, respiratory system and skin.
          May cause sensitization by inhalation.
          S PhraseS: 22-24-26-36/37

          Do not breathe dust.
          Avoid contact with skin.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing and gloves.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage -20°C
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Trypsin, Bovine Pancreas SDS

          Title

          Safety Data Sheet (SDS) 

          Trypsin, Bovine Pancreas Certificates of Analysis

          TitleLot Number
          6502

          References

          Reference overview
          Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.
          Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem. 239, 1799.
          Hummel, B.C.W. 1955. Can. J. Biochem. Physiol. 37, 1393.

          Citations

          Title
        • Elizabeth A. Lipscomb, et al. (2005) The α6β4 integrin maintains the survival of human breast carcinoma cells in vivo. Cancer Research 65, 10970-10976.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-May-2008 RFH
          DescriptionNative trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Has an optimal pH of 8.5-8.8.
          FormSolid
          FormulationSalt-free
          CAS number9002-07-7
          RTECSYN5075000
          EC number3.4.21.4
          ContaminantsChymotrypsin: ≤50 NF units/mg dry weight
          Activity≥2,500 USP units/mg dry weight
          Unit definitionOne USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
          SolubilityHCl (1 mM), H₂O, or Neutral Buffers
          Storage -20°C
          Hygroscopic
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.
          Toxicity Irritant
          Merck USA index14, 9795
          ReferencesBergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.
          Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem. 239, 1799.
          Hummel, B.C.W. 1955. Can. J. Biochem. Physiol. 37, 1393.
          Citation
        • Elizabeth A. Lipscomb, et al. (2005) The α6β4 integrin maintains the survival of human breast carcinoma cells in vivo. Cancer Research 65, 10970-10976.