650277 Trypsin, Bovine, MS Grade

650277
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650277-1SET
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      Description
      OverviewA chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin and 1 ml of resuspension buffer.
      Catalogue Number650277
      Brand Family Calbiochem®
      References
      ReferencesHenzel, W.J., et al. 1993. PNAS 90, 5011.
      Rice, R.H., et al. 1977. Biochim Biophys Acta 492, 316.
      Product Information
      Unit of DefinitionOne BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
      FormLyophilized
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥7500 BAEE U/mg
      Physicochemical Information
      ContaminantsChymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.)
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38-42

      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation.
      S PhraseS: 22-24-26-36/37-45

      Do not breathe dust.
      Avoid contact with skin.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing and gloves.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Trypsin, Bovine, MS Grade SDS

      Title

      Safety Data Sheet (SDS) 

      Trypsin, Bovine, MS Grade Certificates of Analysis

      TitleLot Number
      650277

      References

      Reference overview
      Henzel, W.J., et al. 1993. PNAS 90, 5011.
      Rice, R.H., et al. 1977. Biochim Biophys Acta 492, 316.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2009 JSW
      DescriptionA chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry.
      FormLyophilized
      Recommended reaction conditions
      Materials Provided: • Trypsin, Bovine, MS Grade (Kit Component No. KP31814-20UG): 5 vials, 20 µg each • Trypsin Resuspension Buffer (Kit Component No. KP31815-0.5ML): 1 vial, 0.5 ml Preparation Before Use: Before opening the trypsin centrifuge the tube at 15,000 x g for 5 min. Suspend the MS Grade Trypsin in the Trypsin-Suspension Buffer. Recommended reconstitution concentration 0.5-1 µg/µl. IMPORTANT NOTE: In some cases, after reconstitution, there is insoluble material, which is a non-protein and will not interfere with trypsin activity. If you prefer to remove it, centrifuge the tube briefly and transfer the supernatant to a clean tube. Guidelines for use: For mass spectrometry sequence analysis, MS Grade Trypsin to protein ratio of 1:20 to 1:100 is recommended. Use freshly reconstituted MS Grade Trypsin in any sequence analysis. Any unused trypsin may be stored at -70°C and used within two weeks. Avoid repeated freeze-thaw cycle after re-suspension, which may lead to performance loss. Any available protocol can be used for digestion of proteins with MS Grade Bovine Trypsin.
      ContaminantsChymostrypsin Activity: <3 BTEE U/mg (one BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8.)
      Specific activity≥7500 BAEE U/mg
      Unit definitionOne BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.
      Toxicity Multiple Toxicity Values, refer to MSDS
      ReferencesHenzel, W.J., et al. 1993. PNAS 90, 5011.
      Rice, R.H., et al. 1977. Biochim Biophys Acta 492, 316.