616377 Sigma-AldrichToxin B, Clostridium difficile - Calbiochem

A high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42, by glucosylation of a threonine residue.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Synonyms: Cytotoxin

Overview

Replacement Information

Key Specifications Table

Pricing & Availability

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Description
Overview	A high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42, by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositiol 4,5-bisphosphate. May also block serotonin release, and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
Catalogue Number	616377
Brand Family	Calbiochem®
Synonyms	Cytotoxin

References
References	Mackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685. Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324. Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707. Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.

Product Information
ATP Competitive	N
Form	Lyophilized
Formulation	Lyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
Reversible	N
Quality Level	MQ100

Applications

Biological Information
Biological activity	Cytotoxic as determined in a cell rounding assay using human foreskin cells.
Primary Target	Small GTPases, such as Rho, Rac, and Cdc42
Purity	major band at >200 kDa

Physicochemical Information
Cell permeable	N

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS
RTECS	XW5807300

Safety Information
R Phrase	R: 36/37/38 Irritating to eyes, respiratory system and skin.
S Phrase	S: 36-26 Wear suitable protective clothing. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Irritant
Storage	+2°C to +8°C
Do not freeze	Yes
Special Instructions	Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 2 weeks at 4°C. Toxin B activity will decrease after 2 weeks at 4°C following reconstitution.
Canadian export regulations	Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.

Packaging Information

Transport Information

Supplemental Information

Specifications

Documentation

Toxin B, Clostridium difficile - Calbiochem SDS

Title
Safety Data Sheet (SDS)

Toxin B, Clostridium difficile - Calbiochem Certificates of Analysis

Title	Lot Number
616377	Enter Lot Number

References

Reference overview
Mackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685. Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324. Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707. Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	20-April-2009 JSW
Synonyms	Cytotoxin
Description	A high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42 by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositol 4,5-bisphosphate. May also block serotonin release, and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
Form	Lyophilized
Formulation	Lyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
RTECS	XW5807300
Purity	major band at >200 kDa
Biological activity	Cytotoxic as determined in a cell rounding assay using human foreskin cells.
Solubility	Sterile dH₂O. Please see vial label for lot-specific reconstitution volume.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Special Instructions	Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 2 weeks at 4°C. Toxin B activity will decrease after 2 weeks at 4°C following reconstitution.
Toxicity	Irritant
References	Mackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685. Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324. Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707. Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.