616376 TRAIL, Human, Recombinant, NSO cells

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616376
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616376-10UG
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      Plastic ampoule 10 μg
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      Description
      OverviewRecombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.
      Catalogue Number616376
      Brand Family Calbiochem®
      SynonymsApo2L; TNF-Related Apoptosis-Inducing Ligand
      References
      ReferencesGriffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
      Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
      Pan, G., et al. 1997. Science 277, 815.
      Pan, G., et al. 1997. Science 276, 111.
      Sheridan, J.P., et al. 1997. Science 277, 818.
      Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1 EU/µg TRAIL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      TRAIL, Human, Recombinant, NSO cells SDS

      Title

      Safety Data Sheet (SDS) 

      TRAIL, Human, Recombinant, NSO cells Certificates of Analysis

      TitleLot Number
      616376

      References

      Reference overview
      Griffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
      Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
      Pan, G., et al. 1997. Science 277, 815.
      Pan, G., et al. 1997. Science 276, 111.
      Sheridan, J.P., et al. 1997. Science 277, 818.
      Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.

      Citations

      Title
    • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
    • Merideth C. Kamradt, et al. (2005) The small heat shock protein αB-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3. Journal of Biological Chemistry 280, 11059-11066.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-September-2008 RFH
      SynonymsApo2L; TNF-Related Apoptosis-Inducing Ligand
      DescriptionRecombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.
      FormLyophilized
      FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤1 EU/µg TRAIL
      Biological activityED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
      SolubilityReconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesGriffith, T.S., et al. 1999. J. Exp. Med. 189, 1343.
      Mariani, S.M., et al. 1997. J. Cell Biol. 137, 221.
      Pan, G., et al. 1997. Science 277, 815.
      Pan, G., et al. 1997. Science 276, 111.
      Sheridan, J.P., et al. 1997. Science 277, 818.
      Pitti, R.M., et al. 1996. J. Biol. Chem. 271, 12687.
      Citation
    • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
    • Merideth C. Kamradt, et al. (2005) The small heat shock protein αB-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3. Journal of Biological Chemistry 280, 11059-11066.