654245 TNF-α, Mouse, Recombinant, E. coli

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654245
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654245-10UG
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      Plastic ampoule 10 μg
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      Description
      OverviewRecombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. Referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune systems response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.
      Catalogue Number654245
      Brand Family Calbiochem®
      SynonymsrmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α
      References
      ReferencesScharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
      Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
      Purity≥97% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤100 pg/µg TNF-α
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      TNF-α, Mouse, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      TNF-α, Mouse, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      654245

      References

      Reference overview
      Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
      Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-May-2008 RFH
      SynonymsrmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α
      DescriptionRecombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. It is referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune system's response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.
      FormLyophilized
      FormulationLyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.
      Purity≥97% by SDS-PAGE
      ContaminantsEndotoxin: ≤100 pg/µg TNF-α
      Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
      SolubilityReconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
      Toxicity Standard Handling
      Merck USA index14, 9812
      ReferencesScharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293.
      Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.

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      Categories

      Life Science Research > Proteins and Enzymes > Other Proteins