CL07 TMB Solution

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CL07
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Overview

Replacement Information

Key Specifications Table

Pricing & Availability

Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
CL07-1000ML
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      Glass bottle 1000 ml
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      CL07-100ML
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          Plastic ampoule 100 ml
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          Description
          OverviewA soluble, non-toxic, non-flammable horseradish peroxidase substrate.
          Catalogue NumberCL07
          Brand Family Calbiochem®
          Application Data
          TMB (Soluble Substrate) produces a higher signal with the lowest background and outperforms other commercially available TMB single-component substrates offering the best signal to noise ratio possible.

          Different lots of TMB (Soluble Substrate) were compared in an ELISA containing 5 µg horseradish peroxidase (HRP) conjugate. The absorbance at 620 nm was measured at the indicated times.

          TMB (Soluble Substrate) was stored at 0°C, 4°C, 25°C or 37°C for 30 days and then tested for stability. The following graph demonstrates the stability of TMB (Soluble Substrate) where performance and background absorbance values remain consistent even after 30 days of stress conditions.
          References
          Product Information
          CAS number54827-17-7
          FormLiquid
          FormulationIn a mildly acidic buffer with proprietary stabilizers, pH 3.55 ± 0.20.
          Quality LevelMQ100
          Applications
          Application NotesELISA
          Application CommentsProduct does not contain hazardous ingredients such as polar aprotic solvents (i.e., N,N-Dimethylformamide (DMF) or DMSO) that are found in other TMB formulations. There is also no alcohol in TMB (Soluble Substrate) so it is not flammable and does not require special handling for shipping. Unreacted TMB (Soluble Substrate) should be colorless or a very light-bluish-green prior to use.
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSDV2300000
          Safety Information
          R PhraseR: 21/22-36/38

          Harmful in contact with skin and if swallowed.
          Irritating to eyes and skin.
          S PhraseS: 26-36/37-45

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing and gloves.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Harmful
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          TMB Solution SDS

          Title

          Safety Data Sheet (SDS) 

          TMB Solution Certificates of Analysis

          TitleLot Number
          CL07
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision21-August-2007 RFH
          ApplicationELISA
          Application Data
          TMB (Soluble Substrate) produces a higher signal with the lowest background and outperforms other commercially available TMB single-component substrates offering the best signal to noise ratio possible.

          Different lots of TMB (Soluble Substrate) were compared in an ELISA containing 5 µg horseradish peroxidase (HRP) conjugate. The absorbance at 620 nm was measured at the indicated times.

          TMB (Soluble Substrate) was stored at 0°C, 4°C, 25°C or 37°C for 30 days and then tested for stability. The following graph demonstrates the stability of TMB (Soluble Substrate) where performance and background absorbance values remain consistent even after 30 days of stress conditions.
          DescriptionA soluble, non-toxic, non-flammable horseradish peroxidase substrate.
          BackgroundTMB (Soluble Substrate) is a non-carcinogenic chromophore for use in ELISA and other microwell applications. TMB (Soluble Substrate) contains 3,3',5,5'-tetramethylbenzidine formulated in a mildly acidic buffer with proprietary stabilizers. It is supplied as a one component liquid solution at a ready-to-use working dilution for application use. Unreacted substrate should be colorless or a very light-bluish-green prior to use. The substrate system develops a blue reaction product when reacted with peroxidase conjugates in microwell applications for visual reading at a wavelength of 370 nm and between 620 and 650 nm. For endpoint assays, the addition of an equal volume of 1N or 2N HCl, or 1N H2SO4 stops the reaction and turns the blue reaction product to a yellow reaction product and is read at a wavelength of 450 nm. Endpoint assays should be read between thirty and sixty minutes after addition of stopping solution. TMB (Soluble Substrate) produces a soluble reaction product that is not recommended for membrane or immunohistochemical applications, which require a precipitating reaction product.
          FormLiquid
          FormulationIn a mildly acidic buffer with proprietary stabilizers, pH 3.55 ± 0.20.
          CAS number54827-17-7
          RTECSDV2300000
          CommentsProduct does not contain hazardous ingredients such as polar aprotic solvents (i.e., N,N-Dimethylformamide (DMF) or DMSO) that are found in other TMB formulations. There is also no alcohol in TMB (Soluble Substrate) so it is not flammable and does not require special handling for shipping. Unreacted TMB (Soluble Substrate) should be colorless or a very light-bluish-green prior to use.
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Yes
          Toxicity Harmful