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69522 T7•Tag® Monoclonal Antibody

69522
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69522-3
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      Plastic ampoule 50 μg
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      69522-4
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          Plastic ampoule 250 μg
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          Description
          OverviewThe T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
          SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
          Species/IsotypeMouse monoclonal IgG2b
          Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
          Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
          < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
          FormStabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
          Working dilution1:5,000–1:10,000
          Catalogue Number69522
          Brand Family Novagen®
          References
          References1. Krek, W., et al. 1994. Cell 78,, 161.

          2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.

          3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.
          Product Information
          Components
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          T7•Tag® Monoclonal Antibody Certificates of Analysis

          TitleLot Number
          69522

          References

          Reference overview
          1. Krek, W., et al. 1994. Cell 78,, 161.

          2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.

          3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.

          Brochure

          Title
          Bulk packaging
          Protein Purification and Detection Tools

          Citations

          Title
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        • User Protocols

          Title
          TB015 T7•Tag® Antibody

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