69048 T7•Tag® Antibody HRP Conjugate

69048
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      Plastic ampoule 100 ul
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      Description
      OverviewThe T7•Tag Antibody HRP Conjugate is useful as a single detection reagent and eliminates the need for secondary antibodies or streptavidin conjugates.The enzyme conjugated T7•Tag monoclonal antibody enables detection with only one binding step and eliminates cross-reactivity associated with secondary reagents.

      The T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors).

      This antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase.

      SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
      Species/IsotypeMouse monoclonal IgG2b
      Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
      Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
      < 1 ng (developed with chemiluminescent substrates)
      FormStabilized solution of antibody conjugate in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
      Working dilution1:5,000–1:10,000
      Catalogue Number69048
      Brand Family Novagen®
      References
      Product Information
      Components
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
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      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
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      Supplemental Information
      Specifications

      Documentation

      T7•Tag® Antibody HRP Conjugate Certificates of Analysis

      TitleLot Number
      69048

      Brochure

      Title
      Protein Purification and Detection Tools

      Citations

      Title
    • Niharika B. Mettu, et al. (2007) The nuclear receptor-coactivator interaction surface as a target for peptide antagonists of the peroxisome proliferator-activated receptors. Molecular Endocrinology 21, 2361-2377.
    • Bryan M. Wittmann, Andrea Sherk and Donald P. McDonnell. (2007) Definition of functionally important mechanistic differences among selective estrogen receptor down-regulators. Cancer Research 67, 9549-9560.
    • Debaditya Mukhopadhyay, et al. (2006) SUSP1 antagonizes formation of highly SUMO2/3-conjugated species. Journal of Cell Biology 174, 939-949.
    • Pierre Fotso, et al. (2005) Cog1p plays a central role in the organization of the yeast conserved oligomeric golgi (COG) complex. Journal of Biological Chemistry 280, 27613-27623.
    • Erika Isono, et al. (2005) Functional analysis of Rpn6p, a lid component of the 26S proteasome, using temperature-sensitive rpn6 mutants of the yeast Saccharomyces cerevisiae. Journal of Biological Chemistry 280, 6537-6547.
    • Yoshimitsu Takahashi and Yoshiko Kikuchi. (2005) Yeast PIAS-type Ull1/Siz1 is composed of SUMO ligase and regulatory domains. Journal of Biological Chemistry 280, 35822-35828.
    • Naohide Watanabe and Eric Lam. (2005) Two Arabidopsis metacaspases AtMCP1b and AtMCP2b are arginine/lysine-specific cysteine proteases and activate apoptosis-like cell death in yeast. Journal of Biological Chemistry 280, 14691-14699.
    • Taruho S. Kuroda, Hiroyoshi Ariga and Mitsunori Fukuda. (2003) The actin-binding domain of Slac2-a/melanophilin is required for melanosome distribution in melanocytes. Molecular and Cellular Biology 23, 5245-5255.
    • Ken-Shwo Dai and Choong-Chin Liew. (2001) A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via Its RING domain. Journal of Biological Chemistry 276, 23992-23999.
    • User Protocols

      Title
      TB137 T7•Tag® Antibody HRP Conjugate

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