70043 T7Select® 1-2b DNA

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70043
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      Plastic ampoule 10 μg
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      Description
      OverviewThe T7Select1-2a-c vectors are protein display vectors based on bacteriophage T7. The vector displays an average of 0.1–1 copies of peptides and proteins up to 900 aa in size on the surface of the T7 capsid. Target sequences are fused to the C-terminus of the 10B capsid protein near amino acid 348. T7Select1 phage are grown on a complementing host (BLT5403) that supplies large amounts of the 10A capsid protein from a plasmid. Capsids thus contain mostly 10A protein, along with 0.1–1 copy of 10B fusion protein per virion. The linear genome of T7Select1-2a is 37,245 bp in size. Unique restriction sites are shown on the vector map (TB182).




      This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
      Catalogue Number70043
      Brand Family Novagen®
      References
      Product Information
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
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      Supplemental Information
      Specifications

      Documentation

      T7Select® 1-2b DNA SDS

      Title

      Safety Data Sheet (SDS) 

      T7Select® 1-2b DNA Certificates of Analysis

      TitleLot Number
      70043

      Citations

      Title
    • Stefan Danner and Joel G. Belasco. (2001) T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries. Procedings of the National Academy of Science 98, 12954-12959.
    • User Protocols

      Title
      TB178 T7Select® System Manual

      Vector Map

      Title
      TB182 T7Select 1-2a-c Vector Map

      Vector Sequence

      Title
      T7Select1-2b Sequence

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      Categories

      Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors
      Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors