566660 Smoothened Agonist, SAG - CAS 364590-63-6 - Calbiochem

Overview

Replacement Information

Key Specifications Table

Empirical FormulaCAS #
C₂₈H₂₈ClN₃OS • 2HCl • 2H₂O 364590-63-6

Pricing & Availability

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566660-1MG
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      Glass bottle 1 mg
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      566660-5MG
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          Description
          OverviewA cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM). Shown to induce Smo internalization. Shown to induce Hedgehog pathway activation (EC50 = ∼ 3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations. A 10 mM (500 µg/86 µl) solution of Smoothened Agonist, SAG (Cat. No. 566661) in H2O is also available.
          Catalogue Number566660
          Brand Family Calbiochem®
          SynonymsN-Methyl-Nʹ-(3-pyridinylbenzyl)-Nʹ-(3-chlorobenzo[b]thiophene-2-carbonyl)-1,4-diaminocyclohexane, SAG1.3
          References
          ReferencesWang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
          Meloni, A.R., et al. 2006. Mol. Cell. Biol. 26, 7750.
          Chen, W., et al. 2004. Science 306, 2257.
          Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
          Frank-Kamenetsky, M., et al. 2002. J. Biol. 1, 10.
          Product Information
          CAS number364590-63-6
          FormLight yellow solid
          Hill FormulaC₂₈H₂₈ClN₃OS • 2HCl • 2H₂O
          Chemical formulaC₂₈H₂₈ClN₃OS • 2HCl • 2H₂O
          Hygroscopic Hygroscopic
          Structure formula ImageStructure formula Image
          Quality LevelMQ100
          Applications
          Biological Information
          Purity≥98% by HPLC
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Hygroscopic Hygroscopic
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Smoothened Agonist, SAG - CAS 364590-63-6 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Smoothened Agonist, SAG - CAS 364590-63-6 - Calbiochem Certificates of Analysis

          TitleLot Number
          566660

          References

          Reference overview
          Wang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
          Meloni, A.R., et al. 2006. Mol. Cell. Biol. 26, 7750.
          Chen, W., et al. 2004. Science 306, 2257.
          Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
          Frank-Kamenetsky, M., et al. 2002. J. Biol. 1, 10.

          Citations

          Title
        • Wang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-February-2012 RFH
          SynonymsN-Methyl-Nʹ-(3-pyridinylbenzyl)-Nʹ-(3-chlorobenzo[b]thiophene-2-carbonyl)-1,4-diaminocyclohexane, SAG1.3
          DescriptionA cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM). Shown to induce Smo internalization. Shown to induce Hedgehog pathway activation (EC50 ∼3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations.
          FormLight yellow solid
          Intert gas (Yes/No) Packaged under inert gas
          CAS number364590-63-6
          Chemical formulaC₂₈H₂₈ClN₃OS • 2HCl • 2H₂O
          Structure formulaStructure formula
          Purity≥98% by HPLC
          SolubilityDMSO (10 mg/ml) or H₂O (25 mg/ml)
          Storage +2°C to +8°C
          Hygroscopic
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesWang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.
          Meloni, A.R., et al. 2006. Mol. Cell. Biol. 26, 7750.
          Chen, W., et al. 2004. Science 306, 2257.
          Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA 99, 14071.
          Frank-Kamenetsky, M., et al. 2002. J. Biol. 1, 10.
          Citation
        • Wang, J., et al. 2010. Proc. Natl. Acad. Sci. USA. 107, 9323.