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534036 Smo Antagonist, IHR-NAc - Calbiochem

A cell-permeable, potent and competitive inhibitor of Smo activity that blocks SAG access (IC₅₀ = 19 nM) and SAG-induced Hh pathway (IC₅₀ = 3.1 nM).

Smo Antagonist, IHR-NAc - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 5-Acetamido-2-chloro-N-(4-(2,5-dichlorobenzamido)phenyl)benzamide, SAG-Induced Hh inhibitor, IHR-Nac

Primary Target: Smo
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534036
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₂₂H₁₆Cl₃N₃O₃

Pricing & Availability

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      		 Glass bottle 10 mg
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      Description
      OverviewA cell-permeable 2,5-dichlorobenzamido compound that acts as a potent and competitive inhibitor of Smo activity. Occupies the SAG binding pocket in the hepta-helical domain to block Smoothened (Smo) agonist (SAG) access (IC50 = 19 nM) and blocks SAG-induced Hedgehog pathway (IC50 = 3.1 nM). Basal wild-type Smo activity is also shown to be equally sensitive to its action (IC50 = 14 nM). Blocks Gli2 accumulation at the ciliary tip in response to SAG treatment and reduces Smo-mediated Gli processing and Sufu/Gli interaction in NIH3T3 cells. Also shown to promote a loss of Smo-dependent Gli activity in cells with W535L and L412F oncogenic Smo mutations.
      Catalogue Number534036
      Brand Family Calbiochem®
      Synonyms5-Acetamido-2-chloro-N-(4-(2,5-dichlorobenzamido)phenyl)benzamide, SAG-Induced Hh inhibitor, IHR-Nac
      DescriptionSmo Antagonist, IHR-NAc
      References
      ReferencesFan, C. W., et al. 2014. Chem. Biol. 21, 1680.
      Product Information
      FormWhite solid
      Hill FormulaC₂₂H₁₆Cl₃N₃O₃
      Chemical formulaC₂₂H₁₆Cl₃N₃O₃
      ReversibleY
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetSmo
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Smo Antagonist, IHR-NAc - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Fan, C. W., et al. 2014. Chem. Biol. 21, 1680.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-June-2016 JSW
      Synonyms5-Acetamido-2-chloro-N-(4-(2,5-dichlorobenzamido)phenyl)benzamide, SAG-Induced Hh inhibitor, IHR-Nac
      DescriptionA cell-permeable 2,5-dichlorobenzamido compound that acts as a potent and competitive inhibitor of Smo activity. Occupies the SAG binding pocket in the hepta-helical domain to block Smoothened (Smo) agonist (SAG) access (IC50 = 19 nM) and blocks SAG-induced Hedgehog pathway (IC50 = 3.1 nM). Basal wild-type Smo activity is also shown to be equally sensitive to its action (IC50 = 14 nM). Blocks Gli2 accumulation at the ciliary tip in response to SAG treatment and reduces Smo-mediated Gli processing and Sufu/Gli interaction in NIH3T3 cells. Also shown to promote a loss of Smo-dependent Gli activity in cells with W535L and L412F oncogenic Smo mutations.
      FormWhite solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₂H₁₆Cl₃N₃O₃
      Purity≥98% by HPLC
      SolubilityDMSO (50 mg/ml)
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesFan, C. W., et al. 2014. Chem. Biol. 21, 1680.