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115948 Safranine O (C.I. 50240)

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Replacement Information

Key Specifications Table

CAS #EC NumberHill FormulaMolar Mass
477-73-6 207-518-8 C₂₀H₁₉ClN₄ 350.85 g/mol

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      Glass bottle 25 g
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      Catalogue Number115948
      DescriptionSAFRANINE O (C.I. 50240) FOR MICROSCOPY
      OverviewSafranin O (C.I. 50240) is a CERTISTAIN® dry dye that is used for the preparation of a staining solution for e.g. Gram stain procedures in smears and sections of human origin in bacteriology. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Gram-negative bacteria will be counterstained by Gram's safranin O solution and appear orange.
      As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations.
      Product Information
      CAS number477-73-6
      EC number207-518-8
      Hill FormulaC₂₀H₁₉ClN₄
      Molar Mass350.85 g/mol
      HS Code3204 13 00
      Structure formula ImageStructure formula Image
      Quality LevelMQ300
      Application Certistain® dry dye used for Gram staining
      Biological Information
      Physicochemical Information
      pH value10 (10 g/l, H₂O, 20 °C)
      Bulk density400 kg/m3
      Solubility50 g/l
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Hazard Pictogram(s) Hazard Pictogram(s)
      Hazard Statement(s)H318: Causes serious eye damage.
      Precautionary Statement(s)P280: Wear eye protection.
      P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
      P313: Get medical advice/ attention.
      Signal WordDanger
      Storage class10 - 13 Other liquids and solids
      WGKWGK 2 obviously hazardous to water
      Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
      Safety Information
      Hazard Symbols Hazard SymbolsIrritant
      R PhraseR 41
      Risk of serious damage to eyes.
      S PhraseS 26-39
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.Wear eye/face protection.
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +2°C to +30°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Dye content (spectrophotometrically)≥ 80 %
      Identity (UV/VIS-Spectrum)passes test
      Absorption maximum λ max (ethanol 50 %)530 - 534 nm
      Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %)1260 - 1570
      TLC-Testpasses test
      Loss on drying (110 °C)≤ 15 %
      Suitability for microscopypasses test


      Safranine O (C.I. 50240) SDS


      Safety Data Sheet (SDS) 

      Safranine O (C.I. 50240) Certificates of Analysis

      Product Number PackagingSpecificationLot Number
      1159480025 Glass bottlePDF Specification Document


      HARLECO Dyes and Stains

      Data Sheet

      Technical Data Sheet 1.20589.0500 GranuCult® PEMBA (Polymyxin Pyruvate Egg yolk Mannitol Bromothymolblue Agar) (base) acc. ISO 21871 (MilliporeSigma)

      Instructions for Use

      115948 Safranine O (C.I. 50240) for microscopy Certistain®