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Description
Overview
Ideal for coupling enzymes to antibodies. Contains a cyclohexane bridge, which gives extra stability to the maleimide group. Both enzyme activity and antibody specificity are preserved after coupling.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Irritant
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Following each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid SMCC can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.
D’Souza, S.E., et al. 1988. J. Biol. Chem.263, 3943.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
A water-soluble derivative of SMCC. Ideal for coupling enzymes to antibodies. Contains a cyclohexane bridge, which gives extra stability to the maleimide group. Both enzyme activity and antibody specificity are preserved after coupling. Water-soluble cross-linking reagents help to avoid the use of organic solvents such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) when preparing bioconjugates.
Form
Off-white solid
CAS number
92921-24-9
Chemical formula
C₁₆H₁₇N₂O₉S · Na
Structure formula
Purity
≥94% by HPLC
Solubility
H₂O (2-5 mg/ml)
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following each use, purge the original vial with inert gas and refrigerate (4°C). Unstable in aqueous solution; reconstitute just prior to use. Alternatively, the solid SMCC can be added directly to the sample to minimize hydrolysis. Hydrolysis is more pronounced in dilute protein solutions and can, therefore, be minimized by using more concentrated protein solutions (>5 mg/ml). For storage of stock solutions, reconstitute in anhydrous DMSO.
Toxicity
Irritant
References
D’Souza, S.E., et al. 1988. J. Biol. Chem.263, 3943.
