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03-0099-01 SMC™ Total-AKT1 Immunoassay Kit

96 well assay  
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      Key Specifications Table

      Species ReactivityKey Applications
      H, R, MSingle Molecule Counting (SMC™)
      Catalogue Number03-0099-01
      Trade Name
      • SMC™
      DescriptionSMC™ Total-AKT1 Immunoassay Kit
      OverviewThe SMC™ Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
      Background InformationThe serine-threonine protein kinase AKT family is the primary downstream mediator of the phosphatidylinositol-3-kinase (PI3K) signaling pathway. Members of the family (AKT1, AKT2, and AKT3) require two separate kinases for complete activation. AKT1 phosphorylates mTOR, whose downstream targets include other kinases, cell cycle regulators and transcription factors that are essential for many cell proliferation and survival processes. AKT1 also up-regulates matrix metalloproteinases and is thus implicated in tumor progression. AKT1 and other members of the AKT family have been an important focus in therapeutic development with their broad effects on the PI3K pathway.
      Product Information
      • TOTAL AKT1 Coated Beads
      • Sample Diluent
      • TOTAL AKT1 Detection Antibody
      • Assay Buffer
      • AKT1 Standard
      • AKT1 Detection Antibody Buffer
      • 10X Wash Buffer
      • Elution Buffer B
      • Buffer D
      • SMC™ TOTAL AKT1 Immunoassay Kit Instructions
      Precision, %
      • Low-end CV% Average: 10%
      • Low-end CV% Range: 2 - 15%
      Quality LevelMQ200
      ApplicationThe sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Total AKT-1 Immunoassay Kit unparalleled performance in the quantification of Total AKT-1 in cell lysates.
      Key Applications
      • Single Molecule Counting (SMC™)
      Biological Information
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information

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