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03-0094-00 SMC™ Interleukin 7 (IL-7)

96 well assay  
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      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey Applications
      IL-7 HSingle Molecule Counting (SMC™)
      Catalogue Number03-0094-00
      Trade Name
      • SMC™
      DescriptionSMC™ Interleukin 7 (IL-7)
      OverviewThe SMC™ IL-7 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
      Background InformationInterleukin-7 (IL-7) is an endogenous cytokine responsible for the proliferation of B- and T-lymphocytes. The active human form of IL-7 has 152 aa and is expressed by fibroblasts, stromal, dendritic and endothelial cells. IL-7 often acts in synergy with other cytokines. For example, IL-7 is found to have an additive effect on T-cell activation in combination with IL-12. However, IL-7 is not redundant, as abnormal function of IL-7 has been implicated in immune disorders such as common variable immunodeficiency, HIV and rheumatoid arthritis (RA). Thus, IL-7 is an excellent candidate for therapeutic development because of its crucial role in the modulation of innate immunity. Both activation and suppression of IL-7 may be of value in future therapeutic interventions.
      Product Information
      • IL-7 Coated Beads
      • Standard Diluent
      • IL-7 Detection Antibody
      • Assay Buffer
      • IL-7 Standard
      • 10X Wash Buffer
      • Elution Buffer B
      • Buffer D
      • SMC™ IL-7 Immunoassay Kit Instructions
      Precision, %
      • Low-end CV% Range: 1 - 20%
      • Low-end CV% Average: 15%
      Quality LevelMQ200
      ApplicationThe sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-7 Immunoassay Kit unparalleled performance in the quantification of IL-7 in human EDTA plasma and serum.
      Key Applications
      • Single Molecule Counting (SMC™)
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • IL-7
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information



      Erenna SMC™ IL-7 Immunoassay Kit Instructions

      SMC™ Interleukin 7 (IL-7)


      Safety Data Sheet (SDS) 


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      Technical Info

      96 well Template 2 Up
      96 well Template Single
      Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods (EMD)

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