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03-0183-00 SMC™ Human UCHL1 High Sensitivity Immunoassay Kit

03-0183-00
96 well assay  
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Single Molecule Counting (SMC™)
      Description
      Catalogue Number03-0183-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human UCHL1 High Sensitivity Immunoassay Kit
      OverviewThe SMC™ HumanUCHL1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationUbiquitin carboxy-terminal hydrolase L1 (UCHL1) is a deubiquitinating enzyme that is very commonly expressed in neurons, constituting about 1-2% of protein content in the human brain. While deubiquitinating enzymes are typically involved in the regulation of proteasome-based degradation of unneeded proteins, the true functions of UCHL1 are still being characterized. Meanwhile, the clinical relevance of this analyte has been studied in recent years in the context of neurodegenerative disorders, severe traumatic brain injury, and even myocardial infarction.
      References
      Product Information
      Components
      • Assay Buffer
      • UCHL1 Coated Beads
      • Standard Diluent
      • UCHL1 Detection Antibody
      • UCHL1 Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • The assay variations of SMC™ Human UCHL1 Immunoassay kit were studied using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
      • Mean intra-assay variation was 6%
      • Mean inter-assay variation was 8%
      Applications
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human UCHL1 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of UCHL1 in human serum, plasma, and CSF.
      Key Applications
      • Single Molecule Counting (SMC™)
      Application NotesThis new streamline kit has the following features including:
      • Improved options for sample prep and incubation
      • Lyophilization of the standard provides the kit at one storage condition
      • Increased reagent volumes to accommodate automation
      • Validated to run on both SMCxPRO™ and Erenna®
      Biological Information
      Species Reactivity NoteSpecificity to the following species samples were tested with the follow results:
      • Mouse – 100%
      • Rat – 100%
      • Monkey – 100%
      • Dog – 100%
      • Cat – 100%
      Cross ReactivityCross-reactivity to the following analytes were tested with the following results:
      • UCHL3 – not cross-reactive
      • alpha-synuclein – not cross-reactive
      Accuracy
      • 3 serum samples spiked with average recovery of 92% with a range of 89-95%
      • 4 plasma samples spiked with average recovery of 93% with a range of 78-106%
      • 5 CSF samples spiked with average recovery of 92% with a range of 89-95%
      Physicochemical Information
      Sensitivity
      • Lower Limit of Quantification: 2.60 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications