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03-0088-00 SMC™ Human Tumor Necrosis Factor Alpha (TNF-α)

96 well assay  
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      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey Applications
      TNF-α HSingle Molecule Counting (SMC™)
      Catalogue Number03-0088-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human Tumor Necrosis Factor Alpha (TNF-α)
      OverviewThe SMC™ Human TNF-α Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
      Background InformationTumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine with cytotoxic activity that is primarily produced by macrophages. TNF-α is involved in systemic inflammation and is a member of the group of cytokines that stimulate the acute phase reaction. When administered to animals or humans it causes inflammation, fever, cardiovascular effects, hemorrhage, coagulation, and acute phase responses similar to those seen during acute infections and shock states. By promoting the inflammatory response, TNF-α is associated with many autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, Crohn’s disease, psoriasis, and refractory asthma. TNF-α also plays a role in cancer and regulates apoptotic cell death, cellular proliferation, differentiation, tumorigenesis, and viral replication.
      Product Information
      • TNF-α Coated Beads
      • Standard Diluent
      • TNF-α Detection Antibody
      • Assay Buffer
      • Human TNF-α Standard
      • 10X Wash Buffer
      • Elution Buffer B
      • Buffer D
      • SMC™ Human TNF-α Immunoassay Kit Instructions
      Precision, %
      • Low-end CV% Range: 5 - 9%
      • Low-end CV% Average: 7%
      Quality LevelMQ200
      ApplicationThe sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Human TNF-α Immunoassay Kit unparalleled performance in the quantification of TNF-α in human EDTA plasma.
      Key Applications
      • Single Molecule Counting (SMC™)
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • TNF-α
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information



      Erenna SMC Human TNFa Immunoassay Kit Instructions

      SMC™ Human Tumor Necrosis Factor Alpha (TNF-α)


      Safety Data Sheet (SDS) 


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      Technical Info

      96 well Template 2 Up
      96 well Template Single
      Application Note: Improved performance with conversion from ELISA to an Erenna® Immunoassay
      Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods (EMD)

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