Millipore Sigma Vibrant Logo

03-0052-00 SMC™ Human Interleukin 4 (IL-4)

96 well assay  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
Request Pricing
Limited AvailabilityLimited Availability
Limited Quantities Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service


      Contact Customer Service


      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey Applications
      IL-4 HSingle Molecule Counting (SMC™)
      Catalogue Number03-0052-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human Interleukin 4 (IL-4)
      OverviewThe SMC™ Human IL-4 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
      Background InformationInterleukin-4 (IL-4), is a cytokine that is a key regulator in humoral and adaptive immunity. IL-4 induces differentiation of naive helper T-cells (Th0 cells) to Th2 cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Further, IL-4 controls the production of IgE, expands IL-4 producing T-cell subsets, and stabilizes effector cell functions. IL-4 has great therapeutic potential due to its role in the development of allergic inflammatory responses. IL-4 also has shown to have promise in drug targeting for cancer.
      Product Information
      • IL-4 Coated Beads
      • Standard Diluent
      • IL-4 Detection Antibody
      • Assay Buffer
      • IL-4 Standard
      • SMC™ Human IL-4 Immunoassay Kit Instructions
      • 10X Wash Buffer
      • Elution Buffer B
      • Buffer D
      Precision, %
      • Low-end CV% Range: 2 - 12%
      • Low-end CV% Average: 7%
      Quality LevelMQ200
      ApplicationThe sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human IL-4 Immunoassay Kit unparalleled performance in the quantification of IL-4 in human EDTA plasma.
      Key Applications
      • Single Molecule Counting (SMC™)
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • IL-4
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information



      Erenna SMC™ Human IL-4 Immunoassay Kit Instructions

      SMC™ Human Interleukin 4 (IL-4)


      Safety Data Sheet (SDS) 


      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      SMCxPRO™ Immunoassay System - Sensitivity You can Count On
      Singulex Erenna® Immunoassay System (MilliporeSigma)

      Technical Info

      96 well Template 2 Up
      96 well Template Single
      Application Note: Improved performance with conversion from ELISA to an Erenna® Immunoassay
      Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods (EMD)