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03-0028-00 SMC™ Human Interleukin 1 Beta (IL-1β)

96 well assay  
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      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey Applications
      IL-1β HSingle Molecule Counting (SMC™)
      Catalogue Number03-0028-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human Interleukin 1 Beta (IL-1β)
      OverviewThe SMC™ IL-1β Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ Immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
      Background InformationInterleukin-1 alpha and beta (IL-1α and IL-1β) are pro-inflammatory cytokines involved in immune defense against infection, and are part of the IL-1 superfamily of cytokines. Both IL-1α and IL-1β are produced by macrophages, monocytes and dendritic cells. IL-1 is involved in various immune responses with a primary role in inflammation, making it a target for Rheumatoid Arthritis (RA). IL-1α and IL-1β are produced as precursor peptides, which are proteolytically processed and released in response to cell injury, and thus induce apoptosis. IL-1β production in peripheral tissue has also been associated with hyperalgesia (increased sensitivity to pain) associated with fever.
      Product Information
      • IL-1β Coated Beads
      • Standard Diluent
      • IL-1β Detection Antibody
      • Assay Buffer
      • Human IL-1β Standard
      • SMC™ Human IL-1β Immunoassay Kit Instructions
      • 10X Wash Buffer
      • Elution Buffer B
      • Buffer D
      Precision, %
      • Low-end CV% Range: 2 - 10%
      • Low-end CV% Average: 6%
      Quality LevelMQ200
      ApplicationThe sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human IL-1β Immunoassay Kit unparalleled performance inthe quantification of IL-1β in human EDTA plasma.
      Key Applications
      • Single Molecule Counting (SMC™)
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • IL-1β
      • Minimum of 10 samples spiked with acceptable recovery between 80-120%.
      Physicochemical Information
      Linearity of DilutionMinimum of 10 samples spiked and diluted (at least 3 dilutions) with acceptable recovery between 80-120%.
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information



      Erenna SMC™ Human IL-1β Immunoassay Kit Instructions

      SMC™ Human Interleukin 1 Beta (IL-1β)


      Safety Data Sheet (SDS) 


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      Technical Info

      96 well Template 2 Up
      96 well Template Single
      Application Note: Improved performance with conversion from ELISA to an Erenna® Immunoassay
      Singulex Erenna® single molecule counting immunoassays outperform electrochemiluminescence (ECL) methods (EMD)