565835 SHIP1 Inhibitor, 3AC - Calbiochem

Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₂₇H₄₉N

Pricing & Availability

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565835-10MG
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      Description
      OverviewA cell-permeable steroidal compound that selectively inhibits SHIP1′ polyphosphatase activity toward PI(3,4,5)P3 (IC50 = 10 µM), but not SHIP2 5′ or PTEN′ polyphosphatase activity toward PI(3,4,5)P3 (IC50 > 1 mM). Effectively inhibits SHIP1-mediated immune response both in vitro and in vivo, as well as SHIP1-dependent cancer cell survival. SHIP2 Inhibitor, AS1938909 is also available (Cat. No. 565840).
      Catalogue Number565835
      Brand Family Calbiochem®
      Synonyms3α-Aminocholestane, SH2-domain-containing inositol 5ʹ-phosphatase 1 (SHIP1) Inhibitor, 3AC
      References
      ReferencesBrooks, R., et al. 2010. J. Immunol. 184, 3582.
      Product Information
      FormWhite solid
      Hill FormulaC₂₇H₄₉N
      Chemical formulaC₂₇H₄₉N
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      ApplicationSHIP1 Inhibitor, 3AC, CAS 2206-20-4, is a cell-permeable, selective inhibitor of SHIP1 polyphosphatase activity toward PIP3 (IC50 = 10 µM), but has no effect on SHIP2 5′ or PTEN activity on PIP3.
      Biological Information
      Purity≥98% by 1H NMR
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SHIP1 Inhibitor, 3AC - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      SHIP1 Inhibitor, 3AC - Calbiochem Certificates of Analysis

      TitleLot Number
      565835

      References

      Reference overview
      Brooks, R., et al. 2010. J. Immunol. 184, 3582.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-August-2012 JSW
      Synonyms3α-Aminocholestane, SH2-domain-containing inositol 5ʹ-phosphatase 1 (SHIP1) Inhibitor, 3AC
      DescriptionA cell-permeable steroidal compound that selectively inhibits SHIP1-, but not SHIP2-, catalyzed PI(3,4,5)P3-to-PI(3,4)P2 dephosphorylation (IC50 = 10 µM vs. >1 mM, respectively), while exhibiting little activity against the PTEN-catalyzed PI(3,4,5)P3-to-PI(4,5)P2 conversion (IC50 > 1 mM). 3AC in vivo treatment (2.292 umol/200 µl/mouse/daily i.p. for 7 d) is reported to boost both peripheral blood granulocyte production (by 4- to 5-fold) and the GVHD- (graft-versus-host disease) suppressing Mac1+Gr1+ MIR (myeloid immunoregulatory) cell population in lymphoid tissues (by 5.55- and 11.53-fold in spleen and lymph nodes, respectively), resulting in an impaired splenocyte allogeneic T cell response in MLR (mixed-leukocyte/lymphocyte reaction) assays (by ≥95%). Similarly, 3AC-treated (9.4 µM; 24 h) primary human PBMCs greatly lose their ability in priming allogeneic T cell response in MLR assays (by 74.5%). In addition, a faster blood cell recovery is also observed in mice receiving 3AC (2.292 umol/200 µl/mouse/daily i.p. for 7 d) after sub-lethal irradiation (550 Rads). Selectively inhibits the growth and survival of SHIP1-dependent human KG-1 and murine C1498 myelogenous leukemia lines (IC50 = 11.5 µM), but not the SHIP1-negative human CML K562 and osteosarcoma MG63 lines. SHIP2 Inhibitor, AS1938909 is also available (Cat. No. 565840).
      FormWhite solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₂₇H₄₉N
      Structure formulaStructure formula
      Purity≥98% by 1H NMR
      SolubilityEthanol (100 mg/ml). Not soluble in DMSO.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesBrooks, R., et al. 2010. J. Immunol. 184, 3582.