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14-331 SGK1 (δ1-59, S422D) Protein, active, 6 µg

Active, N-truncated (missing N-terminal 60 amino acids), His-tagged, human SGK1 fusion protein, containing a S422D mutation, for use in Kinase Assays.

SGK1 (δ1-59, S422D) Protein, active, 6 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-331
6 µg  
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      Overview

      Replacement Information
      Description
      Catalogue Number14-331
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionSGK1 (δ1-59, S422D) Protein, active, 6 µg
      OverviewN-truncated (missing Nterminal 60 amino acids), His-tagged, human SGK1 fusion protein, containing a S422D mutation
      References
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActive, N-truncated (missing N-terminal 60 amino acids), His-tagged, human SGK1 fusion protein, containing a S422D mutation, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed in Sf21 cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a serine/threonine protein kinase that is highly similar to the rat serum-and glucocorticoid-induced protein kinase (SGK). This gene was identified in a screen of hepatocellular genes regulated in response to cellular hydration or swelling. Cellular hydration is a catabolic signal, stimulating glycogenolysis and proteolysis, and inhibiting protein and glycogen synthesis. This kinase has been shown to be important in activating certain potassium, sodium, and chloride channels. Expression of this gene in hepatocytes is stimulated by transforming growth factor-beta (TGF-beta) which participates in the pathophysiology of diabetic complications. Since both TGF-beta expression and SGK expression are elevated in diabetic nephropathy, this suggests an involvement of SGK in the development of this condition.
      Gene Symbol
      • SGK
      • SGK1
      Protein TargetSGK1
      Purification MethodNi-NTA agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O00141 # Protein kinase that plays an important role in cellular stress response. Activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. Sustained high levels and activity may contribute to conditions such as hypertension and diabetic nephropathy. Mediates cell survival signals, phosphorylates and negatively regulates pro-apoptotic FOXO3A. Phosphorylates NEDD4L, which leads to its inactivation and to the subsequent activation of various channels and transporters such as ENaC, Kv1.3, or EAAT1.
      SIZE: 431 amino acids; 48942 Da
      SUBUNIT: Interacts with NEDD4 and NEDD4L.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Endoplasmic reticulum. Note=Nuclear, upon phosphorylation.
      TISSUE SPECIFICITY: Expressed in most tissues with highest levels in the pancreas, followed by placenta, kidney and lung.
      PTM: Regulated by phosphorylation. Phosphoinositide 3-kinase (PI3- kinase) pathway promotes phosphorylation at Ser-422 which in turn increases the phosphorylation of Thr-256 by PDPK1. & Ubiquitinated by NEDD4L; which promotes proteasomal degradation. Ubiquitinated by SYVN1 at the endoplasmic reticulum; which promotes rapid proteasomal degradation and maintains a high turnover rate in resting cells.
      SIMILARITY: SwissProt: O00141 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.
      Molecular Weight48kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by Kinase assay
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -70°C
      Packaging Information
      Material Size6 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      14-331 04053252328633

      Documentation

      SGK1 (δ1-59, S422D) Protein, active, 6 µg SDS

      Title

      Safety Data Sheet (SDS) 

      SGK1 (δ1-59, S422D) Protein, active, 6 µg Certificates of Analysis

      TitleLot Number
      SGK1 (S422D), active - 1797670-A 1797670-A
      SGK1 (S422D), active - 1797670-B 1797670-B
      SGK1 (S422D), active - 28638U-G 28638U-G

      References

      Reference overviewApplicationPub Med ID
      Activation of NHE3 by dexamethasone requires phosphorylation of NHE3 at Ser663 by SGK1
      Wang, Dongsheng, et al
      Am J Physiol, Cell Physiol, 289:C802-10 (2005)  2005

      		15888551 15888551
      Cell surface expression of the ROMK (Kir 1.1) channel is regulated by the aldosterone-induced kinase, SGK-1, and protein kinase A
      Yoo, D., et al
      J Biol Chem, 278:23066-75 (2003)  2003

      		Kinase Assay12684516 12684516
      Characterization of the structure and regulation of two novel isoforms of serum- and glucocorticoid-induced protein kinase
      Kobayashi, T., et al
      Biochem J, 344 Pt 1:189-97 (1999)  1999

      		Protein Kinase/Phosphatase Assays10548550 10548550