555466 Sigma-AldrichRho, GST-Fusion, Human, Recombinant, E. coli

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

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Overview

Replacement Information

Pricing & Availability

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Description
Overview	Recombinant, human Rho fused to GST and expressed in E. coli. A small GTPase of the Ras superfamily. Regulates stress fiber formation in response to growth factor stimulation. Useful as a positive control in Western analysis and as an affinity precipitation reagent using immobilized glutathione resin.
Catalogue Number	555466
Brand Family	Calbiochem®

References
References	Ridley, A. 1996. Curr. Biol. 6, 1256. Ridley, A. and Hall, A. 1992. Cell 70, 389.

Product Information
Activity	GTPγS binding ≥400 mmol/mol
Form	Liquid
Formulation	In 20 mM HEPES, 5 mM MgCl₂, 40% glycerol, 0.05% NaN₃, pH 8.0.
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Key Applications	Affinity Precipitation Immunoblotting (Western Blotting)

Biological Information
Purity	≥90% by SDS-PAGE
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	-20°C
Avoid freeze/thaw	Avoid freeze/thaw
Do not freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Documentation

Rho, GST-Fusion, Human, Recombinant, E. coli SDS

Title
Safety Data Sheet (SDS)

Rho, GST-Fusion, Human, Recombinant, E. coli Certificates of Analysis

Title	Lot Number
555466	Enter Lot Number

References

Reference overview
Ridley, A. 1996. Curr. Biol. 6, 1256. Ridley, A. and Hall, A. 1992. Cell 70, 389.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	12-May-2008 JSW
Description	Recombinant, human Rho fused to GST and expressed in E. coli. A small GTPase of the Ras superfamily. Regulates stress fiber formation in response to growth factor stimulation. Useful as a positive control in immunoblotting analysis and as an affinity precipitation reagent using immobilized glutathione resin.
Form	Liquid
Formulation	In 20 mM HEPES, 5 mM MgCl₂, 40% glycerol, 0.05% NaN₃, pH 8.0.
Concentration Label	Please refer to vial label for lot-specific concentration
Purity	≥90% by SDS-PAGE
Activity	GTPγS binding ≥400 mmol/mol
Preservative	≤0.1% sodium azide
Storage	Avoid freeze/thaw -20°C
Do Not Freeze	Ok to freeze
Special Instructions	Following initial thaw, aliquot and freeze (-20°C).
Toxicity	Standard Handling
References	Ridley, A. 1996. Curr. Biol. 6, 1256. Ridley, A. and Hall, A. 1992. Cell 70, 389.