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530353 Regacin - CAS 72836-33-0 - Calbiochem

530353
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Overview

Replacement Information

Key Specifications Table

Empirical FormulaCAS #
C₉H₈ClN₃S₂ 72836-33-0

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      Glass bottle 25 mg
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      Description
      OverviewAn orally bioavailable, non-toxic, thio-thiadiazolamine compound that specifically inhibits the DNA-binding capacity of virulence regulator, RegA (IC50 = 1.7 µM in E. coli strain MC4100 (kfc-lacZ, pACYC184-regA), by interacting with amino acid residues within a conserved region of the DNA-binding domain, but without affecting RegA dimerization. Interacts with the double helix-turn-helix (HTH) domain that results in a loss of binding affinity of RegA for its DNA targets. This interaction appears to involve W188 and R223 residues that are conserved in several RegA homologs including ToxT, AggR, RegR, and Rns. Shown to be highly effective in reducing Citrobacter rodentium colonization when administered perorally to mice (~50 mg/kg) either 15 minutes before or 12 hours after bacterial inoculation. Reduces the ability of Rns and RegR to activate the transcription of their target promoters, but does not affect the activity of AggR and ToxT.

      Please note that the molecular weight for this compound is batch-specific due to variable water content.
      Catalogue Number530353
      Brand Family Calbiochem®
      Synonyms5-((4-Chlorobenzyl)sulfanyl)-1,3,4-thiadiazol-2-amine, Virulence Regulator RegA Inhibitor, NSC-523933
      References
      ReferencesYang, J., et al. 2013. J. Biol. Chem. 288, 31115.
      Product Information
      CAS number72836-33-0
      FormPale yellow powder
      Hill FormulaC₉H₈ClN₃S₂
      Chemical formulaC₉H₈ClN₃S₂
      ReversibleY
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetRegA
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze 9-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Regacin - CAS 72836-33-0 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Yang, J., et al. 2013. J. Biol. Chem. 288, 31115.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-April-2014 JSW
      Synonyms5-((4-Chlorobenzyl)sulfanyl)-1,3,4-thiadiazol-2-amine, Virulence Regulator RegA Inhibitor, NSC-523933
      DescriptionAn orally bioavailable, non-toxic, thio-thiadiazolamine compound that specifically inhibits the DNA-binding capacity of virulence regulator, RegA (IC50 = 1.7 µM in E. coli strain MC4100 (kfc-lacZ, pACYC184-regA), by interacting with amino acid residues within a conserved region of the DNA-binding domain, but without affecting RegA dimerization. Interacts with the double helix-turn-helix (HTH) domain that results in a loss of binding affinity of RegA for its DNA targets. This interaction appears to involve W188 and R223 residues that are conserved in several RegA homologs including ToxT, AggR, RegR, and Rns. Shown to be highly effective in reducing Citrobacter rodentium colonization when administered perorally to mice (~50 mg/kg) either 15 minutes before or 12 hours after bacterial inoculation. Reduces the ability of Rns and RegR to activate the transcription of their target promoters, but does not affect the activity of AggR and ToxT.
      FormPale yellow powder
      Intert gas (Yes/No) Packaged under inert gas
      CAS number72836-33-0
      Chemical formulaC₉H₈ClN₃S₂
      Purity≥95% by HPLC
      SolubilityDMSO (100 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze 9-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesYang, J., et al. 2013. J. Biol. Chem. 288, 31115.