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SCC009 ReNcell® CX Human Neural Progenitor Cell Line & Culture Media Kit

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SCC009
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      Cult
      Description
      Catalogue NumberSCC009
      Brand Family Chemicon®
      Trade Name
      • ReNcell
      • Chemicon
      DescriptionReNcell® CX Human Neural Progenitor Cell Line & Culture Media Kit
      OverviewReNcell® CX Kit contains human neural progenitor cells, optimized maintenance media and freezing media for culture of human neural stem cells. ReNcell® CX Immortalized Cells (SCC007): ReNcell® CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons and glial cells. ReNcell® CX was derived from the cortical region of human fetal brain. Immortalized by retroviral transduction with the c-myc oncogene, this cell line grows rapidly as a monolayer on laminin with a doubling time of 20-30 hours. Karyotype analyses indicate that the ReNcell® CX retains a normal diploid karyotype in culture even after prolonged passage (>45 passages). ReNcell® CX was developed by the ReNeuron Group plc, a biotech company that specializes in using human somatic stem cells for therapeutics. ReNcell® CX may be used for a variety of research applications such as studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions. ReNcell® CX cells have been obtained in a legal and ethical manner, compliant with current local informed consent procedures. ReNcell® NSC Maintenance Medium (SCM005): ReNcell® Neural Stem Cell (NSC) Maintenance Medium is a defined serum-free, growth factor-free medium that has been optimized for the growth and in vitro differentiation of ReNcell immortalized human neural progenitor cells. When used in conjunction with FGF and EGF, the maintenance medium will allow for the proliferation of ReNcell® immortalized VM and CX neural stem cells. Withdrawal of the growth factors from ReNcell® NSC Maintenance Medium will result in the spontaneous differentiation of ReNcell® immortalized neural progenitor cells. ReNcell® NSC Freezing Medium (SCM007): ReNcell® NSC Freezing Medium is qualified for use with ReNcell® immortalized human neural progenitor cell lines, CX (CHEMICON Catalog No. SCC007) and VM (CHEMICON Catalog No. SCC008) cultured in serum-free conditions with ReNcell® NSC Maintenance Medium (CHEMICON Catalog No. SCM005). The optimized formulation allows for consistent cryopreservation and high viability upon thawing and plating.
      References
      Product Information
      Components
      • ReNcell® Neural Stem Cell Freezing Medium (Cat.# SCM007)
      • ReNcell® NSC Maintenance Media (Cat.# SCM005)
      • ReNcell® CX Immortalized Cell Line (Cat.# SCC007)
      Quality LevelMQ100
      Applications
      ApplicationReNcell® CX Kit contains human neural progenitor cells, optimized maintenance media & freezing media for culture of human neural stem cells.
      Key Applications
      • Stem Cell Culture
      Biological Information
      Stem Cell Type
      • Neural Stem Cells
      Cell Line Type
      • Neural Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEach lot of ReNcell® CX cells has been validated for high level of expression of Nestin and Sox 2 and for their self-renewal and multi-lineage differentiation capacities (please refer to datasheet figures). Cells also display normal karyotype as assessed by chromosome spread and tested negative for mycoplasma. Maternal blood has been screened for HIV, HTLV, Hepatitis B and C.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ReNcell® CX Human Neural Progenitor Cell Line & Culture Media Kit SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewApplicationPub Med ID
      Differential development of neuronal physiological responsiveness in two human neural stem cell lines.
      Donato, R; Miljan, EA; Hines, SJ; Aouabdi, S; Pollock, K; Patel, S; Edwards, FA; Sinden, JD
      BMC neuroscience  8  36  2007

      Show Abstract
      Immunocytochemistry17531091 17531091

      Technical Info

      Title
      ReNcell VM Cell Line - A Model of Human Neural Development and Differentiation

      Data Sheet

      Title
      ReNcell¿ CX Human Neural Progenitor Cell Line & Culture Media Kit

      Newsletters / Publications

      Title
      Cellutions Newsletter: 2007, Volume 1
      Cellutions Newsletter: 2008, Volume 1
      Cellutions Newsletter: 2008, Volume 3

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      Kit Component

      Catalog Number Description  
      SCC007 ReNcell® CX Human Neural Progenitor Cell Line Show Pricing & Availability
      SCM005 ReNcell® NSC Maintenance Media Show Pricing & Availability
      SCM007 ReNcell® Neural Stem Cell Freezing Medium Show Pricing & Availability

      Categories

      Life Science Research > Cell Culture and Systems > Cell Lines > Stem Cell Lines