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RI-13K Rat Insulin RIA

RI-13K
250 tubes  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Insulin RRIARadioactive 125I
      Description
      Catalogue NumberRI-13K
      DescriptionRat Insulin RIA
      Background InformationInsulin, a 5.8 kD hormone is secreted by the islet beta cells in the pancreas.
      References
      Product Information
      Detection methodRadioactive 125I
      Performance
      • ED80 = 0.2 ± 0.02 ng/mL
        ED50 = 0.8 ± 0.04 ng/mL
        ED20 = 3.0 ± 0.36 ng/mL
      Precision, %
      • Inter-assay: 8.5–9.4
      • Intra-assay: 1.4–4.6
      Quality LevelMQ100
      Applications
      ApplicationThis radioimmunoassay (RIA) measures & quantifies Insulin for use in Metabolism & Endocrine research.
      Key Applications
      • Radioimmunoassay
      Application NotesOvernight, equilibrium incubation at 4º C. Sample volume 100 μL serum, plasma, or tissue culture media.
      Biological Information
      SpecificityRat Insulin I 100% Rat Insulin II 100% Human Insulin 100% Human Proinsulin 69% Procine Insulin 100% Sheep Insulin 100% Hamster Insulin 100% Mouse Insulin 100% Rat C-Peptide Not Detectabl Glucagon Not Detectable Somatostatin Not Detectable Pancr
      Species Reactivity
      • Rat
      Analytes Available
      • Insulin
      Entrez Gene SummaryAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
      Radioactive Isotype125I-Insulin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
      SIZE: 110 amino acids; 11981 Da
      SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
      SUBCELLULAR LOCATION: Secreted.
      DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
      SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
      Accuracy
      • 85–93%
      Physicochemical Information
      Linearity of Dilution80 -100%
      Standard Curve Range
      • 0.1–10 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size250 tubes
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Rat Insulin RIA Kit

      Rat Insulin RIA SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Maternal high-fat diet programs Wnt genes through histone modification in the liver of neonatal rats.
      Ke-Feng Yang,Wei Cai,Jia-Li Xu,Wen Shi
      Journal of molecular endocrinology  49  2012

      Show Abstract
      22696509 22696509
      Arginine availability modulates arginine metabolism and TNFα production in peritoneal macrophages from Zucker Diabetic Fatty rats.
      Charlotte Breuillard,Linda Belabed,Sandra Bonhomme,Marie-Céline Blanc-Quintin,Nathalie Neveux,Rémy Couderc,Jean-Pascal De Bandt,Luc Cynober,Sylviane Darquy
      Clinical nutrition (Edinburgh, Scotland)  31  2012

      Show Abstract
      22182949 22182949
      Mechanistic modeling of the effects of glucocorticoids and circadian rhythms on adipokine expression.
      Siddharth Sukumaran,William J Jusko,Debra C DuBois,Richard R Almon
      The Journal of pharmacology and experimental therapeutics  337  2011

      Show Abstract
      21398515 21398515
      Different roles of zinc plus arachidonic acid on insulin sensitivity between high fructose- and high fat-fed rats.
      Inkyung Hwang,Taeho Yoon,Changhoon Kim,Byungmann Cho,Sooil Lee,Moon K Song
      Life sciences  88  2011

      Show Abstract
      21167181 21167181
      Feeding behavior and performance of lambs are influenced by flavor diversity.
      Villalba JJ, Bach A, Ipharraguerre IR
      J Anim Sci  2011

      Show Abstract
      21454862 21454862
      Differential muscle gene expression as a function of disease progression in Goto-Kakizaki diabetic rats.
      Nie J, Xue B, Sukumaran S, Jusko WJ, Dubois DC, Almon RR
      Molecular and cellular endocrinology  2011

      Show Abstract
      21356272 21356272
      Effect of high-fat diet on stress responsiveness in borderline hypertensive rats.
      Mitra A, Crump EM, Alvers KM, Robertson KL, Rowland NE
      Stress  14  42-52. Epub 2010 Jul 28.  2011

      Show Abstract
      20666663 20666663
      Glutamate permeability at the blood-brain barrier in insulinopenic and insulin-resistant rats.
      Hawkins RA, Mokashi A, Dejoseph MR, Vina JR, Fernstrom JD
      Metabolism: clinical and experimental  59  258-266  2010

      Show Abstract
      19793593 19793593
      Dietary intervention prior to pregnancy reverses metabolic programming in male offspring of obese rats.
      Zambrano E, Martínez-Samayoa PM, Rodríguez-González GL, Nathanielsz PW
      J Physiol  588  1791-9. Epub 2010 Mar 29.  2010

      Show Abstract
      20351043 20351043
      Hyperphagia induced by sucrose: Relation to circulating and CSF glucose and corticosterone and orexigenic peptides in the arcuate nucleus.
      Gaysinskaya VA, Karatayev O, Shuluk J, Leibowitz SF
      Pharmacol Biochem Behav  2010

      Show Abstract
      21036188 21036188

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      Analyte Quarterly Vol. 2 2020
      ELISAs, RIAs & GyroMark HT Assays
      Protein Detection Platform and Services Brochure

      Technical Info

      Title
      Quality Control Ranges for Catalog # 6000-K Expected Ranges of Each Analyte Using EMD MILLIPORE RIA Kits as Listed Below

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits