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RI-13K Rat Insulin RIA

250 tubes  
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      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Insulin RRIARadioactive 125I
      Catalogue NumberRI-13K
      DescriptionRat Insulin RIA
      Background InformationInsulin, a 5.8 kD hormone is secreted by the islet beta cells in the pancreas.
      Product Information
      Detection methodRadioactive 125I
      • ED80 = 0.2 ± 0.02 ng/mL
        ED50 = 0.8 ± 0.04 ng/mL
        ED20 = 3.0 ± 0.36 ng/mL
      Precision, %
      • Inter-assay: 8.5–9.4
      • Intra-assay: 1.4–4.6
      Quality LevelMQ100
      ApplicationThis radioimmunoassay (RIA) measures & quantifies Insulin for use in Metabolism & Endocrine research.
      Key Applications
      • Radioimmunoassay
      Application NotesOvernight, equilibrium incubation at 4º C. Sample volume 100 μL serum, plasma, or tissue culture media.
      Biological Information
      SpecificityRat Insulin I 100% Rat Insulin II 100% Human Insulin 100% Human Proinsulin 69% Procine Insulin 100% Sheep Insulin 100% Hamster Insulin 100% Mouse Insulin 100% Rat C-Peptide Not Detectabl Glucagon Not Detectable Somatostatin Not Detectable Pancr
      Species Reactivity
      • Rat
      Analytes Available
      • Insulin
      Entrez Gene SummaryAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
      Radioactive Isotype125I-Insulin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
      SIZE: 110 amino acids; 11981 Da
      SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
      DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
      SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
      • 85–93%
      Physicochemical Information
      Linearity of Dilution80 -100%
      Standard Curve Range
      • 0.1–10 ng/mL
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size250 tubes
      Transport Information
      Supplemental Information



      Protocol: Rat Insulin RIA Kit

      Rat Insulin RIA SDS


      Safety Data Sheet (SDS) 


      Reference overviewPub Med ID
      Arginine availability modulates arginine metabolism and TNFα production in peritoneal macrophages from Zucker Diabetic Fatty rats.
      Charlotte Breuillard,Linda Belabed,Sandra Bonhomme,Marie-Céline Blanc-Quintin,Nathalie Neveux,Rémy Couderc,Jean-Pascal De Bandt,Luc Cynober,Sylviane Darquy
      Clinical nutrition (Edinburgh, Scotland)  31  2012

      Show Abstract
      22182949 22182949
      Maternal high-fat diet programs Wnt genes through histone modification in the liver of neonatal rats.
      Ke-Feng Yang,Wei Cai,Jia-Li Xu,Wen Shi
      Journal of molecular endocrinology  49  2012

      Show Abstract
      22696509 22696509
      Different roles of zinc plus arachidonic acid on insulin sensitivity between high fructose- and high fat-fed rats.
      Inkyung Hwang,Taeho Yoon,Changhoon Kim,Byungmann Cho,Sooil Lee,Moon K Song
      Life sciences  88  2011

      Show Abstract
      21167181 21167181
      Differential muscle gene expression as a function of disease progression in Goto-Kakizaki diabetic rats.
      Nie J, Xue B, Sukumaran S, Jusko WJ, Dubois DC, Almon RR
      Molecular and cellular endocrinology  2011

      Show Abstract
      21356272 21356272
      Mechanistic modeling of the effects of glucocorticoids and circadian rhythms on adipokine expression.
      Siddharth Sukumaran,William J Jusko,Debra C DuBois,Richard R Almon
      The Journal of pharmacology and experimental therapeutics  337  2011

      Show Abstract
      21398515 21398515
      Feeding behavior and performance of lambs are influenced by flavor diversity.
      Villalba JJ, Bach A, Ipharraguerre IR
      J Anim Sci  2011

      Show Abstract
      21454862 21454862
      Effect of high-fat diet on stress responsiveness in borderline hypertensive rats.
      Mitra A, Crump EM, Alvers KM, Robertson KL, Rowland NE
      Stress  14  42-52. Epub 2010 Jul 28.  2011

      Show Abstract
      20666663 20666663
      FoxM1 Is Up-Regulated by Obesity and Stimulates {beta}-Cell Proliferation.
      Davis DB, Lavine JA, Suhonen JI, Krautkramer KA, Rabaglia ME, Sperger JM, Fernandez LA, Yandell BS, Keller MP, Wang IM, Schadt EE, Attie AD
      Mol Endocrinol  2010

      Show Abstract
      20660304 20660304
      Basal Plasma Levels of Insulin, Leptin, Ghrelin, and Amylin Do Not Signal Adiposity in Rats Recovering from Forced Overweight.
      Gloy VL, Lutz TA, Langhans W, Geary N, Hillebrand JJ
      Endocrinology  2010

      Show Abstract
      20668029 20668029
      Circadian variations in gene expression in rat abdominal adipose tissue and relationship to physiology.
      Sukumaran S, Xue B, Jusko WJ, Dubois DC, Almon RR
      Physiol Genomics  42  141-52. Epub 2010 Aug 3.  2010

      Show Abstract
      20682845 20682845


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      Technical Info

      Quality Control Ranges for Catalog # 6000-K Expected Ranges of Each Analyte Using EMD MILLIPORE RIA Kits as Listed Below

      Data Sheet

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      Life Science Research > Protein Detection and Quantification > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits