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03-115 RIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal

03-115
10 assays  10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent upon biological context).
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H, M, RRIP, WB, FC, IH(P)
      Description
      Catalogue Number03-115
      Brand Family Upstate
      Trade Name
      • RIPAb+
      • Upstate
      DescriptionRIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal
      OverviewRIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
      Alternate Names
      • musashi 2
      • musashi homolog 2 (Drosophila)
      Background InformationIn mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. MSI2 or Musashi2 is a member of an RNA-binding protein family that appears to play a central role in post transcriptional gene regulation. The MSI2 gene encodes a protein containing two conserved tandem RNA recognition motifs. MSI2 may have a unique role that is required for the generation and/or maintenance of specific neuronal lineages.
      References
      Product Information
      FormatUnpurified
      Control
      • Includes negative control rabbit IgG antibody and control primers specific for human PTP4A1.
      PresentationAnti-Musashi 2 (Rabbit Monoclonal). One vial containing 50 μL of Rabbit Monoclonal Antibody in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Store at -20°C.

      Normal Rabbit IgG. One vial containing 125 μg of Rabbit IgG in 125 μL of storage buffer containing 0.05% sodium azide.
      Store at -20°C.

      RIP Primers, PTP4A1. One vial containing 75 μL of 5 μM of each primer specific for human PTP4A1. Store at -20°C.
      FOR: ATC CAA CCA ATG CGA CCT TA
      REV: AAG GCC AAT CAA GAA CAT GG
      Applications
      ApplicationThis RIPAb+ Musashi 2 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • RNA Binding Protein Immunoprecipitation (RIP)
      • Western Blotting
      • Flow Cytometry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunoprecipitation from RIP lysate:
      Representative lot data.
      RIP lysate from SHSH-5Y cells (2 X 10E6 cell equivalents per IP) was subjected to immunoprecipitation using 0.5 µL of either a normal rabbit IgG or 0.5 µL of Anti-Musashi 2 antibody.
      Precipitated proteins (lane 1: rabbit IgG, lane 2: anti-Musashi 2) and SHSH-5Y whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Musashi 2 antibody (1:2,000 dilution). Proteins were visualized using One-Step™ IP-Western kit.
      Arrow indicates Musashi 2 (Please see figures).

      Immunohistochemistry (Paraffin) Analysis:
      Representative lot data.
      A previous lot was used in immunohistochemical staining of paraffin-embedded human placenta tissue using 1:100-1:250 dilution of anti-Musashi 2 (Please see figures).

      Western Blot Analysis:
      Representative lot data.
      A 1:1,000-2,000 dilution of a previous lot of this antibody was used to detect Musashi 2 in human brain (A), rat brain (B), SW480 (C), and T47D (D) cell lysates (Please see figures).

      Flow Cytometry: A 1:30 dilution of a previous lot was used in flow cytometry.
      Biological Information
      ImmunogenSynthetic peptide corresponding to N-terminus of human Musashi-2.
      EpitopeN-terminus
      HostRabbit
      SpecificityRecognizes Musashi-2 at and around the N-terminus.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene.
      Gene Symbol
      • FLJ36569
      • MGC3245
      • MSI2H
      • Musashi-2
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity.
      SUBCELLULAR LOCATION: Cytoplasm. Note: Associated with polysomes By similarity.
      TISSUE SPECIFICITY: Ubiquitous; detected at low levels.
      INDUCTION: Up-regulated in astrocytes after brain injury By similarity.
      PTM: Phosphorylated By similarity.
      INVOLVEMENT IN DISEASE: Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
      SEQUENCE SIMILARITIES: Belongs to the Musashi family.
      Contains 2 RRM (RNA recognition motif) domains.
      Molecular Weight~35 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRNA Binding Protein Immunoprecipitation:
      RIP Lysate prepared from SHSH-5Y cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µL of either a normal rabbit IgG or 5 µL of Anti-Musashi 2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
      Successful immunoprecipitation of Musashi 2-associated RNA was verified by qPCR using RIP Primers PTP4A1 (Please see figures).
      Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size10 assays
      Material Package10 assays per set. Recommended use: ~5 μL of antibody per RIP (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      RIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal SDS

      Title

      Safety Data Sheet (SDS) 

      RIPAb+ Musashi 2 - RIP Validated Antibody and Primer Set, rabbit monoclonal Certificates of Analysis

      TitleLot Number
      RIPAb+ Musashi 2 - 2388306 2388306
      RIPAb+ Musashi 2 - 2212011 2212011
      RIPAb+ Musashi 2 - 2490683 2490683
      RIPAb+ Musashi 2 - NG1940123 NG1940123
      RIPAb+ Musashi 2 - NRG1761590 NRG1761590
      RIPAb+ Musashi 2 -2496172 2496172

      References

      Reference overviewPub Med ID
      Musashi2 sustains the mixed-lineage leukemia-driven stem cell regulatory program.
      Park, SM; Gönen, M; Vu, L; Minuesa, G; Tivnan, P; Barlowe, TS; Taggart, J; Lu, Y; Deering, RP; Hacohen, N; Figueroa, ME; Paietta, E; Fernandez, HF; Tallman, MS; Melnick, A; Levine, R; Leslie, C; Lengner, CJ; Kharas, MG
      J Clin Invest  125  1286-98  2015

      Show Abstract
      25664853 25664853
      Optimisation of ICP-MS collision/reaction cell conditions for the determination of elements likely to be interfered (V, Cr, Fe, Co, Ni, As and Se) in foodstuffs.
      Ali Kadar,Laurent Noël,Rachida Chekri,Christelle Vastel,Sandrine Millour,Thierry Guérin
      Talanta  85  2011

      Show Abstract
      21962690 21962690