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03-184 RIPAb+™ LSM14A - RIP Validated Antibody and Primer Set

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03-184
10 assays  10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      M, H, RRIP, WB
      Description
      Catalogue Number03-184
      Trade Name
      • RIPAb+
      • Upstate
      DescriptionRIPAb+™ LSM14A - RIP Validated Antibody and Primer Set
      OverviewRIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
      The LSM14A protein is an Sm-like protein. These proteins contain the Sm sequence motif, which consists of two regions separated by a linker region that varies in length and folds as a loop. The Sm-like proteins appear to form a stable heteromer detected in tri-snRNP particles, which are important for pre-mRNA splicing.
      Alternate Names
      • LSM14 homolog A
      • LSM14 homolog A (SCD6, S. cerevisiae)
      • LSM14A, SCD6 homolog A (S. cerevisiae)
      • Protein FAM61A
      • Protein SCD6 homolog
      • Putative alpha-synuclein-binding protein
      • RNA-associated protein 55
      • chromosome 19 open reading frame 13
      • family with sequence similarity 61, member A
      Background InformationThe LSM14A protein is an Sm-like protein. These proteins have been identified in a variety of organisms based on sequence homology with the members of the Sm protein family. These proteins contain the Sm sequence motif, which consists of two regions separated by a linker region that varies in length and folds as a loop. The Sm-like proteins appear to form a stable heteromer detected in tri-snRNP particles, which are important for pre-mRNA splicing.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Includes negative control normal rabbit IgG antibody and control primers specific for the human LSM14A.
      PresentationAnti-LSM14A (rabbit polyclonal), . One vial containing 50 µg affinity purified polyclonal in buffer containing 0.1 M Tris-glycine, 0.150 M NaCl, pH 7.4, and 0.05% sodium azide before the addition of 30% glycerol. Store at -20°C.
      Normal Rabbit IgG. One vial containing 125 µg of rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
      RIP Primers, U6snRNA.
      One vial containing 75 μL of 5 μM of each primer specific for human U6 snRNA. Store at -20°C.
      FOR: CTC GCT TCG GCA GCA CA
      REV: AAC GCT TCA CGA ATT TGC GT
      Quality LevelMQ100
      Applications
      ApplicationThis RIPAb+ LSM14A -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • RNA Binding Protein Immunoprecipitation (RIP)
      • Western Blotting
      Application NotesImmunoprecipitation from RIP lysate:
      Representative lot data.
      RIP lysate from 293 cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, (Cat. #PP64B), or 5 µg of Anti-LSM14A antibody (Cat. # CS204313). Ten percent of the precipitated proteins (lane 1: rabbit IgG, lane 2: LSM14A) and 293 whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-LSM14A antibody (Cat. # CS204313, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00231)
      Arrow indicates LSM14A.
      Western Blot Analysis:
      Representative lot data.
      NIH/3T3 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-LSM14A (1:1000) dilution.
      Proteins were visualized using a Donkey Anti-Rabbit conjugated to HRP and a chemiluminescence detection system.
      Arrow indicates LSM14A (~58 kDa).
      Biological Information
      ImmunogenGST-tagged recombinant protein corres-ponding to the entire protein of human LSM14A.
      EpitopeUnknown
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes full-length LSM14A.
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySm-like proteins were identified in a variety of organisms based on sequence homology with the Sm
      protein family (see SNRPD2; 601061). Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.[supplied by OMIM]
      Gene Symbol
      • AlphaSNBP
      • C19orf13
      • DKFZP434D1335
      • DKFZp434D1335
      • FAM61A
      • RAP55
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryPTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
      SEQUENCE SIMILARITIES:Belongs to the LSM14 family.

      Molecular Weight~58 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRNA Binding Protein Immunoprecipitation:
      Representative lot data.
      RIP Lysate prepared from 293 cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal rabbit IgG, or 5 µg Anti-LSM14A antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
      Successful immunoprecipitation of LSM14A-associated RNA was verified by qPCR using RIP Primers, U6snRNA,(Figure 1).
      Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size10 assays
      Material Package10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications