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03-251 RIPAb+™ IGF2BP2 Antibody

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03-251
10 assays  10 assays per set. Recommended use: 1.25 μg of antibody per RIP (dependent upon biological context).
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      HWB, RIP
      Description
      Catalogue Number03-251
      Trade Name
      • RIPAb+
      • Upstate
      DescriptionRIPAb+™ IGF2BP2 Antibody
      OverviewRIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA-binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
      IGF2BP2 (Insulin-like growth factor 2 mRNA-binding protein 2) is a member of the IGF-II mRNA-binding protein family that post-transcriptionally regulates IGF2 during developmental stages. IGF2BP1, 2, and 3 bind the UTR region of IGF2 transcripts, and are implicated in the transport of RNA targets, to enable protein synthesis at specific locations in the cytoplasm. Research has shown that the IGF2 gene may be involved in ovarian cancer.
      Alternate Names
      • Insulin-like growth factor 2 mRNA-binding protein 2
      • IGF2 mRNA-binding protein 2
      • IMP-2
      • Hepatocellular carcinoma autoantigen p62
      • IGF-II mRNA-binding protein 2
      • VICKZ family member 2
      Background InformationInsulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a member of the IGF-II mRNA binding protein family that posttranscriptionally regulates IGF2 during developmental stages. IGF2 mRNA-binding protein family members (IGF2BP1, 2 and 3) bind UTR region of IGF2 transcripts and are implicated in the transport of RNA targets to enable protein synthesis at specific locations in the cytoplasm. Studies have indicated variations in the IGF2BP2 gene may affect fetal glucose metabolism and plays a role in type 2 diabetes. In addition to playing a role in glucose metabolism and dieabetes, the insulin-like growth factor (IGF) signaling axis plays an important role in cancer biology. Research has shown IGF2 to be emerging as an important gene for ovarian cancer.
      References
      Product Information
      FormatPurified
      Control
      • Includes normal mouse IgG and primers specific for human IGF2 mRNA.
      PresentationAnti-IGF2BP2 (Mouse Monoclonal). One vial containing 12.5 µg protein G purified monoclonal in buffer containing 0.1 M Tris-glycine (pH 7.4), 150mM NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Store at -20° C.
      Concentration: 0.176 mg/mL
      Normal mouse IgG. One vial containing 125 µg purified mouse IgG in 125 µL storage buffer containing 0.1% sodium azide. Store at -20°C.
      RIP Primer IGF2. One vial containing 75 μL of 5 μM of each primer specific for human IGF2 mRNA. Store at -20°C.
      FOR: GCG GCT TCT ACT TCA GCA G
      REV: CAG GTG TCA TAT TGG AAG AAC
      Quality LevelMQ100
      Applications
      ApplicationIGF2BP2 (Insulin-like growth factor 2 mRNA-binding protein 2) is a member of the IGF-II mRNA-binding protein family that post-transcriptionally regulates IGF2 during developmental stages.
      Key Applications
      • Western Blotting
      • RNA Binding Protein Immunoprecipitation (RIP)
      Application NotesWestern Blot Analysis:
      Representative lot data.
      HepG2 cell lysate was probed with Anti-IGF2BP2, clone 5E10-3E1 (1:20,000 dilution).
      Proteins were visualized using a Goat Anti-Mouse IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
      Arrow indicates IGF2BP2 (~66 kDa).
      Note: An uncharacterized band appears at ~50 kDa in some lysates.
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human IGF2BP2
      Clone5E10-3E1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
      Gene Symbol
      • IGF2BP2
      • IMP2
      • VICKZ2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.

      SUBUNIT STRUCTURE: Interacts with HNRPD and IGF2BP1.

      SUBCELLULAR LOCATION: Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.

      TISSUE SPECIFICITY: Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.

      DOMAIN: The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.

      MISCELLANEOUS: Autoantibodies against IGF2BP2 are detected in sera from some patients with hepatocellular carcinoma.

      SEQUENCE SIMILARITIES: Belongs to the RRM IMP/VICKZ family.

      Contains 4 KH domains.

      Contains 2 RRM (RNA recognition motif) domains.

      SEQUENCE CAUTION: The sequence AAH21290.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~66 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRNA Binding Protein Immunoprecipitation:
      Representative lot data
      RIP Lysate prepared from 293 cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 1.25 µg of either a normal mouse IgG or 1.25 µg Anti-IGF2BP2 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
      Successful immunoprecipitation of IGF2BP2-associated RNA was verified by qPCR using RIP Primers IGF2, (Figure 1).
      Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size10 assays
      Material Package10 assays per set. Recommended use: 1.25 μg of antibody per RIP (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      RIPAb+™ IGF2BP2 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      RIPAb+™ IGF2BP2 Antibody Certificates of Analysis

      TitleLot Number
      Monoclonal Antibody/Primer Set_2833939 2833939
      RIPAb+ IGF2BP2 - Q1999625 Q1999625
      RIPAb+™ IGF2BP2
      RIPAb+™ IGF2BP2 2890932
      RIPAb+™ IGF2BP2 - 2559240 2559240
      RIPAb+™ IGF2BP2 - 3229614 3229614
      RIPAb+™ IGF2BP2 - 3506767 3506767
      RIPAb+™ IGF2BP2 - 3793160 3793160
      RIPAb+™ IGF2BP2 -2752496 2752496
      RIPAb+™ IGF2BP2 -2793776 2793776

      References

      Reference overviewApplicationPub Med ID
      RNA-binding proteins regulate the expression of the immune activating ligand MICB.
      Nachmani, D; Gutschner, T; Reches, A; Diederichs, S; Mandelboim, O
      Nature communications  5  4186  2014

      Show Abstract
      Western Blotting24924487 24924487
      Oncogenic NRAS, required for pathogenesis of embryonic rhabdomyosarcoma, relies upon the HMGA2-IGF2BP2 pathway.
      Li, Z; Zhang, Y; Ramanujan, K; Ma, Y; Kirsch, DG; Glass, DJ
      Cancer Res  73  3041-50  2013

      Show Abstract
      23536553 23536553

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      Categories

      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies
      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > RNA-Binding Protein Immunoprecipitation (RIP) > RIP Validated Antibodies