552095 R-Spondin 4, Mouse, Recombinant

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552095
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Overview

Replacement Information

Key Specifications Table

Purity
≥95% by SDS-PAGE

Pricing & Availability

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552095-25UG
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      Glass bottle 25 μg
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      Description
      OverviewRecombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling.
      Catalogue Number552095
      Brand Family Calbiochem®
      SynonymsCristin 4
      References
      ReferencesHendrickx, M. and L. Leyns. 2008. Develop. Growth Differ. 50, 229.
      Ishii, Y., et al. 2008. J. Invest. Dermatol. 128, 867.
      Kim, K.A., et al. 2008. Mol. Biol. Cell 19, 2588.
      Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA 104 14700.
      Nam, J.S., et al. 2007. Gene Expr. Patterns 7, 306.
      Pemberton, T.J., et al. 2007. Dev. Dyn. 236, 2245.
      Bergmann, C., et al. 2006. Am. J. Hum. Genet. 79, 1105.
      Blaydon, D.C., et al. 2006. Nat. Genet. 38, 1245.
      Nam, J.S., et al. 2006. J. Biol. Chem. 281, 13247.
      Product Information
      FormSolid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      R-Spondin 4, Mouse, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      R-Spondin 4, Mouse, Recombinant Certificates of Analysis

      TitleLot Number
      552095

      References

      Reference overview
      Hendrickx, M. and L. Leyns. 2008. Develop. Growth Differ. 50, 229.
      Ishii, Y., et al. 2008. J. Invest. Dermatol. 128, 867.
      Kim, K.A., et al. 2008. Mol. Biol. Cell 19, 2588.
      Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA 104 14700.
      Nam, J.S., et al. 2007. Gene Expr. Patterns 7, 306.
      Pemberton, T.J., et al. 2007. Dev. Dyn. 236, 2245.
      Bergmann, C., et al. 2006. Am. J. Hum. Genet. 79, 1105.
      Blaydon, D.C., et al. 2006. Nat. Genet. 38, 1245.
      Nam, J.S., et al. 2006. J. Biol. Chem. 281, 13247.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2011 RFH
      SynonymsCristin 4
      DescriptionRecombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling.
      FormSolid
      FormulationLyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotoxin: <1.0 EU/µg protein
      Biological activityED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.
      SolubilityReconstitute to 200 µg/ml in PBS.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesHendrickx, M. and L. Leyns. 2008. Develop. Growth Differ. 50, 229.
      Ishii, Y., et al. 2008. J. Invest. Dermatol. 128, 867.
      Kim, K.A., et al. 2008. Mol. Biol. Cell 19, 2588.
      Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA 104 14700.
      Nam, J.S., et al. 2007. Gene Expr. Patterns 7, 306.
      Pemberton, T.J., et al. 2007. Dev. Dyn. 236, 2245.
      Bergmann, C., et al. 2006. Am. J. Hum. Genet. 79, 1105.
      Blaydon, D.C., et al. 2006. Nat. Genet. 38, 1245.
      Nam, J.S., et al. 2006. J. Biol. Chem. 281, 13247.