ECM650 Quantitative Pseudopodia Assay Kit

ECM650
1 kit  
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      Overview

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      Description
      Catalogue NumberECM650
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionQuantitative Pseudopodia Assay Kit
      OverviewChemotaxis, or directed cell movement is a fundamental cellular process, necessary for embryonic development, angiogenesis, wound healing, and immune responses, as well as pathological events associated with inflammation and cancer metastasis [Lauffenburger & Horwitz, 1996]. Prior to cell body translocation or chemotaxis, a cell must first extend a dominant pseudopodium (lamellipodium) in the direction of the chemoattractant gradient [Lauffenburger & Horwitz, 1996; Parent & Devreotes, 1999]. Once a dominant pseudopodium is formed, cell movement can begin in the direction of the gradient. Formation of a directional pseudopodium is tightly controlled by signal transduction mechanisms that temporally and spatially regulate changes in focal adhesions, microtubules, and the actin/myosin cytoskeleton [Ridley et al., 1992; Schmidt et al., 1993; Manes et al., 1999; Nobes & Hall, 1999; Eddy et al., 2000].

      The CHEMICON Quantitative Pseudopodia Assay Kit is a novel system designed to specifically isolate and quantitate extending or retracting pseudopodia from the cell body [Cho & Klemke, 2002]. This assay provides a quick and efficient system to aid in measuring the effects of various natural or synthetic compounds that specifically impact the microenvironment of the pseudopodial organelle including signaling processes, integrins or other adhesion receptors, and unique gene products. The assay is ideal for screening pharmacological agents that either stimulate or inhibit pseudopodia formation as well as for the identification of novel drug targets that impact cell chemotaxis.

      In addition, Chemicon also provides QCM™ 24-well insert cell migration assay systems, CytoMatrix™ Cell Adhesion strips coated with ECM proteins or anti integrin antibodies, QCM Chemotaxis 96-well cell migration assay, and QuantiMatrix™ ECM protein ELISA kits

      For Research Use Only; Not for use in diagnostic procedures
      Materials Required but Not Delivered· Desired Extracellular Matrix (ECM) Protein such as fibronectin, collagen or vitronectin

      · Tissue Culture Dishes and Supplies, including at least 2 additional 24 well plates

      · Tissue Culture Media (appropriate complete media (with serum) for culturing cells)

      · Migration Media (appropriate media for culturing cells without serum; supplemented with 0.1-0.2% BSA)

      · Low Speed centrifuge for harvesting cells

      · Hemacytometer for cell counting

      · 37o C (~6% CO2) Incubator

      · 1X PBS

      · Protein Lysis Buffer

      · BCA Microprotein Assay System (Pierce)

      · Microplate reader
      References
      Product Information
      Components
      • Pseudopodia Quantification Inserts - (Part No. 71611) - 1 X 24 well plate containing 12 6.5 micrometer transwells.
      • Cottons Swabs - (Part No. 10202) - 14 swabs
      • Pseudopodia Stain Solution - (Part No. 71605) 20 mL
      • Forceps - (Part No.10203) 1 pair of forceps
      • Pseudopodia Stain Extraction Buffer - (Part No. 71604) 20 mL
      Applications
      ApplicationThe Quantitative Pseudopodia Assay Kit is a novel system designed to specifically isolate & quantitate extending or retracting pseudopodia from the cell body.
      Key Applications
      • Activity Assay
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore Kit and Materials at 2-8ºC until their expiration date
      Packaging Information
      Material Size1 kit
      Transport Information
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      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Migration Assays