ECM660 Pseudopodia Purification Kit

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ECM660
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      Overview

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      Key Specifications Table

      Key Applications
      ACT
      Description
      Catalogue NumberECM660
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionPseudopodia Purification Kit
      OverviewChemotaxis, or directed cell movement is a fundamental cellular process, necessary for embryonic development, angiogenesis, wound healing, and immune responses, as well as pathological events associated with inflammation and cancer metastasis [Lauffenburger & Horwitz, 1996]. Prior to cell body translocation or chemotaxis, a cell must first extend a dominant pseudopodium (lamellipodium) in the direction of the chemoattractant gradient [Lauffenburger & Horwitz, 1996; Parent & Devreotes, 1999]. Once a dominant pseudopodium is formed, cell movement can begin in the direction of the gradient. Formation of a directional pseudopodium is tightly controlled by signal transduction mechanisms that temporally and spatially regulate changes in focal adhesions, microtubules, and the actin/myosin cytoskeleton [Ridley et al., 1992; Schmidt et al., 1993; Manes et al., 1999; Nobes & Hall, 1999; Eddy et al., 2000].

      The CHEMICON Pseudopodia Purification Kit is a novel system designed to specifically isolate and purify extending pseudopodia proteins from the cell body [Cho & Klemke, 2002]. This Kits provides a quick and efficient system to purify pseudopodia proteins and their responses to various natural or synthetic compounds that specifically impact the microenvironment of the pseudopodial organelle including signaling processes, integrins or other adhesion receptors, and unique gene products. This kit provides a novel method for pseudopodia protein purification; identify signaling complex formation as a result of chemotaxis stimuli.

      For Research Use Only; Not for use in diagnostic procedures
      Materials Required but Not Delivered· Desired Extracellular Matrix (ECM) protein such as fibronectin, collagen, or vitronectin

      · Tissue Culture Dishes and Supplies and at least 2 - 6 Well Plates

      · Tissue Culture Media (appropriate complete media (with serum) for culturing cells)

      · Migration Media (appropriate media for culturing cells without serum; supplemented with 0.1-0.2% BSA)

      · Low Speed centrifuge for harvesting cells

      · Hemacytometer for cell counting

      · 37°C (~6% CO2) Incubator

      · 1X PBS

      · Trypsin

      · Methanol

      · Laboratory Tissue (delicate task wipes)

      · Parafilm

      · BCA Microprotein Assay System (Pierce)

      · Microplate reader
      References
      Product Information
      Components
      • Pseudopodia Purification Inserts - (Catalog No. 71608) 1 X 6 well plate containing 6 X 24mm 3.0-micrometer transwells.
      • Western Blot Lysis Buffer - (Catalog No. 71606) 20 mL of 50 mM Tris, pH 7.4, 5 mM EDTA, 150 mM NaCl, 1% SDS
      • Immunoprecipitation Buffer - (Catalog No. 71601) 20 mL of 50 mM Tris, pH 7.4, 5 mM EDTA, 150 mM NaCl, 1% Triton-X-100
      • Cell Scrapers - (Catalog No.71603) 3 each
      • Forceps - (Catalog No. 10203) 1 each
      • Cotton Swabs - (Catalog No. 10202) 14 swabs
      Quality LevelMQ100
      Applications
      ApplicationThe Pseudopodia Purification Kit is a novel system designed to specifically isolate & purify extending pseudopodia proteins from the cell body.
      Key Applications
      • Activity Assay
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore Kit and Materials at 2-8ºC until their expiration date
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Migration Assays