539779 ProteoExtract® Complete Mammalian Proteome Extraction Kit

539779
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539779-1KIT
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      Description
      OverviewA complete mammalian proteome extraction kit (C-PEK) designed to extract virtually all proteins from both mammalian tissues and culture cells in a simple two step process within a microcentrifuge tube to minimize protein loss. The extraction results in a total protein sample that is a clarified and non-viscous protein solution showing higher resolution and an increased total number of spots in 2-D gel electrophoresis. Sample size: 1x107 - 1x108 cells or 50-100 mg tissue.
      Catalogue Number539779
      Brand Family Calbiochem®
      SynonymsC-PEK Mammalian Kit
      Materials Required but Not Delivered High purity water, conductivity <5 µS (e.g., )
      Cell culture equipment, media etc. for cell growth (e. g., RPMI, DMEM).
      Micropipettes and tips, 10 µl, 200 µl and 1 ml size (e.g., Eppendorf, Gilson or equivalent)
      Cooled centrifuge and rotor for 50 ml tube size (Eppendorf, Heraeus, Nalgen, etc.)
      Cooled microcentrifuge and rotor up to 25,000 x g for 2 ml tube size (Eppendorf, Heraeus, Nalgen etc.)
      Thermomixer or rolling facility (e.g., Eppendorf)
      Optional: Ultracentrifuge and rotor up to 75,000 x g for 2 ml tube size (e.g. Beckman, Sorvall etc.)
      For tissue extraction: Mortar and pestle made from porslin, small spatula. These instruments must be cooled to -70°C or colder prior to be used in the C-PEK procedure!
      References
      ReferencesYuan, X., et al. 2002. Electrophoresis 23, 1185.
      Allen, L. 2000. Functional Genomics Nature 405 819.
      Herbert, B. 1999. Electrophoresis 20, 660.
      Product Information
      Form20 Extractions
      Kit containsWash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase® Nuclease (Cat. No. 71206), Glass Beads, and a user protocol.
      Applications
      Biological Information
      Sample TypeMammalian tissues and cultured cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Intended useProteoExtract™ Complete Mammalian Proteome Extraction Kit is designed to extract total proteins from mamalian tissue culture cells or mammalian tissue.
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage +2°C to +8°C
      Storage Conditions Wash Buffer
      The Wash Buffer is provided ready-to-use. The buffer should be stored at 4°C.

      Resuspension Buffer
      The Resuspension Buffer is provided ready-to-use. It can be stored at 4°C up to 6 month. For prolonged storage freeze the buffer in convenient aliquots at -20°C.
      Avoid repeated freezing and thawing!

      Extraction Reagent
      The Extraction Reagent is supplied as dry powder and can be stored unopened at 4°C. The Extraction Reagent should be reconstituted just prior to use as described below. After reconstitution, it can be stored at 4°C up to 6 month. For prolonged storage freeze the buffers in convenient aliquots at -20°C.
      Avoid repeated freezing and thawing!

      Reducing Agent
      The Reducing Agent is supplied as dry powder. It can be stored unopened at 4°C. The Reducing Agent should be reconstituted just prior to use as described below. After reconstitution, aliquot the unused material in 60 µl volumes and freeze at -20°C for further use.
      Avoid repeated freezing and thawing!

      Benzonase®
      Benzonase® should be stored at 4°C.
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Kit containsWash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase® Nuclease (Cat. No. 71206), Glass Beads, and a user protocol.
      Specifications

      Documentation

      ProteoExtract® Complete Mammalian Proteome Extraction Kit Certificates of Analysis

      TitleLot Number
      539779

      References

      Reference overview
      Yuan, X., et al. 2002. Electrophoresis 23, 1185.
      Allen, L. 2000. Functional Genomics Nature 405 819.
      Herbert, B. 1999. Electrophoresis 20, 660.

      Technical Info

      Title
      Western Blotting Quick Reference Product Guide

      User Protocols

      Title
      User Protocol